Intercellular Ca2+ waves in rat heart muscle
Intercellular Ca2+ waves in rat heart muscle
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DOI：
10.1111/j.1469-7793.1998.669bd.x
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发表时间：
1998-11-01
影响因子：
5.5
通讯作者：
Wier, WG
中科院分区：
文献类型：
--
作者：
Lamont, C;Luther, PW;Wier, WG
1.使用共聚焦激光扫描显微镜观察微注射钙指示剂fluo-3.2的完整大鼠心室梁中Ca 2+波的细胞间传输。Ca ~（2+）波通常不能在细胞间传递。在确定的个人端到端的细胞接触，成功的传输穿插失败，这有时会发生，尽管一个明显的小spritz的Ca 2+细胞之间。细胞间传播的概率（P-tran）为0.13.3。Ca 2+波优先出现在连接细胞的连接处，在连接蛋白-43处，而在酶促分离的心肌细胞中则随机出现.β-肾上腺素能刺激显著增加P-tran（至0.22），而间隙连接通道的解偶联剂庚醇显著降低P-tran（至0.045）。在因损伤而导致[Ca 2 +]（1）高的区域，随着每个细胞-细胞连接的经过，波频率显着降低。6.心肌间隙连接的Ca 2+渗透性可能受到调节，心肌间隙连接传递Ca 2+的能力较低可能有助于控制Ca 2+从受损区域的扩散。
1. Confocal laser scanning microscopy was used to visualize intercellular transmission of Ca2+ waves in intact rat ventricular trabeculae micro-injected with the calcium indicator fluo-3.2. Ca2+ waves usually failed to be transmitted from cell to cell. At identified individual end-to-end cell contacts, successful transmission interspersed with failure, which sometimes occurred despite an apparent small spritz of Ca2+ between cells. The probability of cell to cell transmission (P-tran) was 0.13.3. Ca2+ waves arose preferentially near junctions of connected cells, where connexin-43 was found, but randomly in enzymatically disconnected heart cells.4. beta-Adrenergic stimulation significantly increased P-tran (to 0.22) and heptanol, an uncoupler of gap junction channels, significantly decreased it (to 0.045).5. In regions of high [Ca2+](1) due to damage, wave frequency decreased markedly with each cell-cell junction passed.6. The Ca2+ permeability of cardiac gap junctions may be regulated, and the low ability of cardiac gap junctions to transmit Ca2+ may help control the spread of Ca2+ from damaged regions.