Effects of sodium ascorbate (vitamin C) and 2‐methyl‐1,4‐naphthoquinone (vitamin K3) treatment on human tumor cell growth in vitro. I. Synergism of combined vitamin C and K3 action
Effects of sodium ascorbate (vitamin C) and 2‐methyl‐1,4‐naphthoquinone (vitamin K3) treatment on human tumor cell growth in vitro. I. Synergism of combined vitamin C and K3 action
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DOI：
10.1002/1097-0142(19890301)63:5
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发表时间：
1989-03
期刊：
影响因子：
6.2
通讯作者：
V. Noto;H. Taper;Jiang Yi-hua;J. Janssens;J. Bonté;W. De Loecker
中科院分区：
文献类型：
--
作者：
V. Noto;H. Taper;Jiang Yi-hua;J. Janssens;J. Bonté;W. De Loecker
研究了单独或联合施用抗坏血酸钠（维生素 C）和 2-甲基-1,4-萘醌（维生素 K3）对体外培养的人肿瘤细胞系 MCF-7（乳腺癌）、KB（口腔表皮样癌）和 AN3-CA（子宫内膜腺癌）的影响。当单独给药时，维生素 C 或 K3 仅在高浓度（分别为 5.103 μmol/1 和 105 nmol/1）时才具有生长抑制作用。两种维生素的联合给药在低 10 至 50 倍的浓度下显示出对细胞生长的协同抑制作用。在这个水平上单独给予维生素是没有毒性的。三种细胞系对此处理的敏感性略有不同，KB 系稍高。在含有维生素 C 和 K3 的培养基中添加过氧化氢酶可以完全抑制这种肿瘤细胞生长抑制作用，这表明过氧化氢的过量产生暗示了导致上述作用的机制。
The effects of sodium ascorbate (vitamin C) and 2‐methyl‐1,4‐naphthoquinone (vitamin K3) administered separately or in combination on the in vitro cultured human neoplastic cell lines MCF‐7 (breast carcinoma), KB (oral epidermoid carcinoma), and AN3‐CA (endometrial adenocarcinoma) have been examined. When given separately, vitamin C or K3 had a growth inhibiting action only at high concentrations (5.103 μmol/1 and 105 nmol/1, respectively). Combined administration of both vitamins demonstrated a synergistic inhibition of cell growth at 10 to 50 times lower concentrations. At this level separately given vitamins are not toxic. The sensitivity to this treatment was somewhat different in the three cell lines, being slightly higher for KB line. This tumor cell growth inhibitory effect was completely suppressed by the addition of catalase to the culture medium containing vitamins C and K3, suggesting an excessive production of hydrogen peroxide as being implied in mechanisms responsible for the above‐mentioned effects.