PE_PGRS proteins are differentially expressed by Mycobacterium tuberculosis in host tissues
PE_PGRS proteins are differentially expressed by Mycobacterium tuberculosis in host tissues
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DOI：
10.1016/j.micinf.2006.03.015
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发表时间：
2006-07-01
影响因子：
5.8
通讯作者：
Fadda, Giovanni
中科院分区：
文献类型：
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作者：
Delogu, Giovanni;Sanguinetti, Maurizio;Fadda, Giovanni
PE-PGRS基因表达的特征将有助于确定该蛋白家族在结核分枝杆菌生物学中的作用。在本报告中，采用定量实时RT-PCR（QRT-PCR）来评估三种PEPGRS基因（rv 0746，rv 1651 c和rv 1818 c）在不同实验条件下的表达。三个PE-PGRS基因在无菌培养中表现出相似的表达谱，在对数生长后期和稳定期早期出现显著上调。rv 1651 c基因表达在骨髓源性巨噬细胞的细胞内生长后增加，但在II型人肺细胞中不增加，而rv 0746在两种体外系统中均被诱导。小鼠感染M.在小鼠结核病的慢性阶段，rv 1651 c和rv 0746的表达水平在脾组织中最高，分别上调> 20倍和> 30倍。在同一时间段内，肺中的表达水平保持较低。在不同的实验条件下，rv 1818 c基因的表达没有显著变化。本研究结果表明，M.结核分枝杆菌对PE-PGRS基因的表达具有差异调节作用，rv 0746和rv 1651 c等基因的表达受到显著诱导，而M.结核病在宿主细胞和组织中持续存在。(c)2006年，Elsevier SAS。All rights reserved.
Characterization of PE-PGRS gene expression will help define the role of this protein family in the biology of Mycobacterium tuberculosis. In this report, quantitative real-time RT-PCR (QRT-PCR) was implemented to assess expression of three PEPGRS genes (rv0746, rv1651c and rv1818c) under different experimental conditions. The three PE-PGRS genes showed a similar expression profile in axenic cultures, with a significant up-regulation occurring at late log and early stationary phases. rv1651c gene expression increased following intracellular growth in bone marrow-derived macrophages but not in type-II human pneumocytes, while rv0746 was induced in both in vitro systems. Following the infection of mice with M. tuberculosis, expression levels of rv1651c and rv0746 normalized to fitsZ and 16S rRNA were highest in the spleen tissue during the chronic stages of murine tuberculosis, with a > 20- and > 30-fold up-regulation, respectively. Levels of expression remained lower in the lung over the same time period. Expression of the rv1818c gene did not change significantly under different experimental conditions tested. The results of this study indicate that M. tuberculosis can differentially regulate expression of PE-PGRS genes and that genes such as rv0746 and rv1651c are significantly induced while M. tuberculosis persists in host cells and tissues. (c) 2006 Elsevier SAS. All rights reserved.