Functional regions of the presynaptic cytomatrix protein Bassoon: significance for synaptic targeting and cytomatrix anchoring
Functional regions of the presynaptic cytomatrix protein Bassoon: significance for synaptic targeting and cytomatrix anchoring
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DOI：
10.1016/s1044-7431(03)00015-0
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发表时间：
2003-06-01
影响因子：
3.5
通讯作者：
Gundelfinger, ED
中科院分区：
文献类型：
--
作者：
Dresbach, T;Hempelmann, A;Gundelfinger, ED
突触囊泡的神经递质胞外分泌被限制在突触前质膜的特定部位称为活动区。一个复杂的细胞基质的蛋白质专门组装在活性区，CAZ，被认为形成一个分子支架，组织神经递质释放位点。在此，我们分析了CAZ的主要支架蛋白Bassoon的突触靶向和细胞基质关联。通过结合免疫细胞化学和转染培养的海马神经元，我们发现巴松管的中心部分在突触靶向和CAZ关联中起关键作用。我们的数据提供了巴松管中存在不同功能区域的第一个实验证据，并表明位于中心的CAZ靶向功能可能与靶向膜结合的突触细胞器的n端能力相补充。(C) 2003 Elsevier Science（美国）版权所有。
Exocytosis of neurotransmitter from synaptic vesicles is restricted to specialized sites of the presynaptic plasma membrane called active zones. A complex cytomatrix of proteins exclusively assembled at active zones, the CAZ, is thought to form a molecular scaffold that organizes neurotransmitter release sites. Here, we have analyzed synaptic targeting and cytomatrix association of Bassoon, a major scaffolding protein of the CAZ. By combining immunocytochemistry and transfection of cultured hippocampal neurons, we show that the central portion of Bassoon is crucially involved in synaptic targeting and CAZ association. An N-terminal region harbors a distinct capacity for N-myristoylation-dependent targeting to synaptic vesicle clusters, but is not incorporated into the CAZ Our data provide the first experimental evidence for the existence of distinct functional regions in Bassoon and suggest that a centrally located CAZ targeting function may be complemented by an N-terminal capacity for targeting to membrane-bounded synaptic organelles. (C) 2003 Elsevier Science (USA). All rights reserved.