Recognition of foot-and-mouth disease virus and its capsid protein VP1 by bovine peripheral T lymphocytes
Recognition of foot-and-mouth disease virus and its capsid protein VP1 by bovine peripheral T lymphocytes
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DOI：
10.1099/0022-1317-77-4-727
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发表时间：
1996-04-01
影响因子：
3.8
通讯作者：
Parkhouse, RME
中科院分区：
文献类型：
--
作者：
GarciaValcarcel, M;Doel, T;Parkhouse, RME
与体液免疫反应相比，T细胞在口蹄疫病毒免疫中的作用仍然不清楚。本文系统、纵向研究了牛外周血T淋巴细胞对口蹄疫病毒及其亚单位蛋白VP1的细胞识别。用单一病毒血清型多次接种可诱导血清型交叉反应性增殖T细胞谱系，该谱系在个体动物之间以及所使用疫苗的血清型之间的程度有所不同。接种疫苗和急性感染牛的原发性增殖T细胞应答相对于加强免疫后相同动物测定的应答幅度较弱。相比之下，初次和二次暴露于病毒后产生的循环抗体水平良好。免疫或感染牛的淋巴细胞表型分析显示，与免疫相比，感染后CD8（+）T细胞略有增加。然而，一般来说，循环淋巴细胞的特征是相似的。因此，我们无法使用增殖或表型分析来区分接种牛和恢复期牛。多重免疫牛对VP1的T细胞识别具有型别特异性，这意味着用全病毒观察到的交叉反应性应答可能归因于VP1以外的蛋白质。与其他研究相反，用重组VP1免疫仅诱导低水平的中和抗体，并且即使在两次免疫后也未能引起深刻的增殖反应或保护。
The role of T cells in immunity to foot-and-mouth disease virus is still poorly defined compared to that of the humoral response. In this paper we describe a systematic, longitudinal study on the cellular recognition of FMDV and its subunit protein VP1 by bovine peripheral blood T lymphocytes. Multiple vaccination with a single virus serotype induced a serotype crossreactive proliferative T cell repertoire that varied in magnitude between individual animals and with the serotype of the vaccine used. Primary proliferative T cell responses of vaccinated and acutely infected cattle were weak relative to the magnitude of responses determined for the same animals after boosting. In contrast, the level of circulating antibody produced after both primary and secondary exposure to virus was good. Phenotypic analysis of lymphocytes from vaccinated or infected cattle showed a small increase in CD8(+) T cells after infection compared to vaccination. However, in general the profiles of circulating lymphocytes elicited were similar. Thus, we were not able to use proliferative or phenotypic analyses to distinguish between vaccinated and convalescent cattle. T cell recognition of VP1 by multiply-vaccinated cattle was serotype-specific implying that the cross-reactive responses observed with whole virus may be attributed to proteins other than VP1. In contrast to other studies, immunization with recombinant VP1 induced only low levels of neutralizing antibody and failed to elicit profound proliferative responses or protection even after two immunizations.