A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers
A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers
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负责 Friend 和 Moloney 逆转录病毒增强子不同组织特异性的 DNA 元件
DOI：
10.1128/jvi.62.2.614-618.1988
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发表时间：
1988
影响因子：
5.4
通讯作者：
P. Charnay
中科院分区：
文献类型：
--
作者：
H. Thiesen;Z. Bösze;L. Henry;P. Charnay
莫洛尼鼠肉瘤病毒（MuSV）和莫洛尼鼠白血病病毒（MuLV）的转录增强子具有与Friend MuLV不同的细胞类型特异性。虽然这三种增强子在红系细胞中的活性大致相等，但Moloney MuSV和Moloney MuLV增强子在T淋巴细胞中的活性是Friend MuLV增强子的20至40倍。使用突变增强子，我们已经表明，Moloney MuSV和Friend MuLV增强子的核苷酸序列之间的特定差异是它们在T细胞中不同活性的原因。我们的数据允许定位的DNA元件，在增强子内重复几次，它调节T细胞中的增强子的活性，而不影响它在红系细胞中。因此，该元件似乎是增强子的组织特异性的决定因素之一。
The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.