Selective oxidation of 5-hydroxymethylcytosine with micelle incarcerated oxidants to determine it at single base resolution.
Selective oxidation of 5-hydroxymethylcytosine with micelle incarcerated oxidants to determine it at single base resolution.
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DOI：
10.1016/j.bmcl.2015.11.017
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发表时间：
2015-12
影响因子：
2.7
通讯作者：
S. Fukuzawa;K. Tachibana;S. Tajima;I. Suetake
中科院分区：
文献类型：
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作者：
S. Fukuzawa;K. Tachibana;S. Tajima;I. Suetake
5-甲基胞嘧啶（5 mC）被10 - 11易位（泰特）酶氧化。这一过程之后，胸腺嘧啶DNA糖基化酶被认为是甲基胞嘧啶去甲基化的机制。5-羟甲基胞嘧啶（5 hmC）是去甲基化过程中最重要的关键氧化代谢产物之一，因此需要简单准确的单碱基分辨率测定5 hmC的方法。在本研究中，我们开发了一种温和的催化氧化的5-hmC使用胶束嵌顿氧化剂，使确定的位置5 hmC在单碱基分辨率。
5-Methylcytosine (5mC) is oxidized by ten-eleven translocation (TET) enzymes. This process followed by thymine DNA glycosylase is proposed to be the mechanism for methylcytosine demethylation. 5-Hydroxymethylcytosine (5hmC) is one of the most important key oxidative metabolites in the demethylation process, and therefore, simple and accurate method to determine 5hmC at single base resolution is desired. In the present study, we developed a mild catalytic oxidation of 5-hmC using micelle incarcerated oxidants that enables to determine the position of 5hmC at single base resolution.