A gravity-induced flow injection system for surface plasmon resonance biosensor.
A gravity-induced flow injection system for surface plasmon resonance biosensor.
复制标题
DOI：
10.1016/j.talanta.2013.03.037
复制
发表时间：
2013-08
期刊：
影响因子：
6.1
通讯作者：
Chao Zhou;Ying Mu;Mengchao Yang;Q. Song;Ying Zhang;Zhongyu Wu;Liancheng Xiang;W. Jin;Q. Jin
中科院分区：
文献类型：
--
作者：
Chao Zhou;Ying Mu;Mengchao Yang;Q. Song;Ying Zhang;Zhongyu Wu;Liancheng Xiang;W. Jin;Q. Jin
许多便携式表面等离子共振 (SPR) 设备已被开发用于现场护理 (POC) 测试。同时，微型泵已被制造成集成到这些设备中以进行流动注射分析（FIA）。然而，（微型）泵、管道及其外部控制单元非常占用空间。在这里，我们开发了一种基于重力诱导流动注射（gFI）系统的用于SPR检测的无动力流动注射分析（FIA）方法。 gFI 系统是无内胎的，不需要控制。流体通过其自身的重力被驱动到检测区域。使用过渡通道来增加入口储液器和出口储液器之间的液位差。将液体样品放入入口储存器后，液体样品的流速在过渡通道中增加。在到达传感表面之前，样品的流速是稳定的（误差小于10%）。流速的波动对 SPR 响应信号有影响，该信号已使用内部参考成功去噪。借助gFI系统，SPR成像生物传感器能够手动进行实时检测。成功获得了DNA杂交和蛋白质固定化的SPR响应。
A number of portable surface plasmon resonance (SPR) devices have been developed for point-of-care (POC) testing. Meanwhile, micropumps have been fabricated to be integrated into these devices for flow injection analysis (FIA). However, the (micro) pumps, the tubes and their external control units were space-consuming. Here we developed a power-free flow injection analysis (FIA) method for SPR detection based on a gravity-induced flow injection (gFI) system. The gFI system was tubeless and did not need to be controlled. The fluid was driven into the detection areas by its own gravitational force. A transition channel was used to increase the liquid-level difference between the inlet reservoir and the outlet reservoir. After a liquid sample was placed in the inlet reservoir, the flow rate of the liquid sample was increased in the transition channel. Before it arrived at the sensing surface, the flow rate of the sample was steady (with an error of less than 10%). The fluctuation of the flow rate had an influence on the SPR response signal, which was successfully denoised using an internal reference. With the gFI system, the SPR imaging biosensor was able to perform real-time detection manually. The SPR responses of DNA hybridization and protein immobilization were successfully obtained.