Cloning and characterisation of the abundant cytoplasmic 7S RNA from mouse cells.
Cloning and characterisation of the abundant cytoplasmic 7S RNA from mouse cells.
复制标题
小鼠细胞中丰富的细胞质 7S RNA 的克隆和表征。
DOI：
10.1093/nar/10.14.4259
复制
发表时间：
1982
影响因子：
14.9
通讯作者：
G. Birnie
中科院分区：
文献类型：
--
作者：
A. Balmain;R. Krumlauf;J. Vass;G. Birnie
从小鼠胚胎小RNA制备cDNA文库，并筛选与高度丰富的细胞质7S RNA互补的克隆的存在。选择一个克隆（pA 6），其在从在高分辨率聚丙烯酰胺/尿素凝胶上运行的细胞质RNA制备的北方印迹上仅与7S RNA杂交。序列分析表明，该克隆的7S RNA的5'端至少有65个核苷酸与高度重复的小鼠B1家族具有广泛的同源性。克隆的小鼠7S序列与从大鼠、人和鸡细胞制备的RNA之间的异源杂交表明，7S RNA分子的非B1部分在最近的真核生物进化过程中高度保守。在小鼠、人类、大鼠和鸡细胞的基因组中存在多个7S RNA基因拷贝，但这些生物体中的拷贝数和基因组组织存在实质性差异。
A cDNA library has been prepared from mouse embryo small RNAs and screened for the presence of clones complementary to the highly abundant cytoplasmic 7S RNA. One clone (pA6) was selected which hybridized exclusively with 7S RNA on a Northern blot prepared from cytoplasmic RNA run on high resolution polyacrylamide/urea gels. Sequence analysis of this clone has shown that at least 65 nucleotides at the 5' end of 7S RNA are extensively homologous with the highly repeated mouse B1 family. Heterologous hybridisations between the cloned mouse 7S sequence and RNAs prepared from rat, human and chick cells have shown that the non-B1 part of the 7S RNA molecule has been highly conserved during recent eucaryotic evolution. There are multiple copies of 7S RNA genes in the genomes of mouse, human, rat and chick cells, but substantial differences exist in copy number and genomic organisation in these organisms.