Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora
Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora
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DOI：
10.1016/j.febslet.2014.11.045
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发表时间：
2015-01-02
期刊：
影响因子：
3.5
通讯作者：
Nishihara, Masahiro
中科院分区：
文献类型：
--
作者：
Sasaki, Nobuhiro;Nishizaki, Yuzo;Nishihara, Masahiro
测定了日本龙胆（Gentiana triflora）叶片中积累的黄酮类化合物主要为异荭草苷（木犀草素6- c -葡萄糖苷）和异荭草苷4′-葡萄糖苷。分离到一段编码udp -葡萄糖依赖性葡萄糖基转移酶的cDNA (GtUF6CGT1)，该酶参与c -糖基黄酮的生物合成。重组GtUF6CGT1蛋白可以通过c -连锁将葡萄糖基团转移到黄酮骨架的C6位置，以udp -葡萄糖为葡萄糖基供体。这些c -糖基黄酮也在花瓣中积累。GtUF6CGT1的表达与叶片和花瓣中c -糖基黄酮的积累具有良好的相关性。GtUF6CGT1是第一个报道的介导黄酮骨架直接c -葡萄糖基化的c -葡萄糖基转移酶。(C) 2014年欧洲生化学会联合会。Elsevier B.V.版权所有。
The major flavonoids accumulated in leaves of Japanese gentian (Gentiana triflora) were determined as isoorientin (luteolin 6-C-glucoside) and isoorientin 4'-glucoside. A cDNA (GtUF6CGT1) was isolated that encoded the UDP-glucose-dependent glucosyltransferase that is involved in C-glycosylflavone biosynthesis. The recombinant GtUF6CGT1 protein could transfer a glucose group to the C6 position of a flavone skeleton through C-linkage, using UDP-glucose as the glucosyl donor. These C-glycosylflavones also accumulated in petals. A good correlation was observed between GtUF6CGT1 expression and C-glycosylflavone accumulation in leaves and petals. GtUF6CGT1 is the first reported C-glucosyltransferase that mediates direct C-glucosylation of the flavone skeleton. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.