BUTYRATE EFFECTS ON GROWTH, MORPHOLOGY, AND FIBRONECTIN PRODUCTION IN PC-3 PROSTATIC-CARCINOMA CELLS
BUTYRATE EFFECTS ON GROWTH, MORPHOLOGY, AND FIBRONECTIN PRODUCTION IN PC-3 PROSTATIC-CARCINOMA CELLS
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DOI：
10.1002/pros.2990120109
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发表时间：
1988-01-01
期刊：
影响因子：
2.8
通讯作者：
ESPEVIK, T
中科院分区：
文献类型：
--
作者：
HALGUNSET, J;LAMVIK, T;ESPEVIK, T
丁酸钠（NaBT）在几种转化细胞系中诱导分化。本文报道了NaBT对人前列腺癌细胞PC-3中一些转化相关参数的影响。NaBT对细胞增殖产生可逆的抑制作用，但非锚定生长比单层生长更敏感。软琼脂糖菌落在0.1 mM时减少了50%以上，这个浓度几乎不影响固体基质上的生长。单层细胞对NaBT的反应是扩散和变平，这是由光显微镜和电子显微镜相结合的形态测量技术所证明的。暴露于2mm NaBT 4天后，平均细胞覆盖的基质面积约为对照细胞覆盖面积的两倍。然而，平均细胞体积保持不变。这种扁平化与应力纤维数量的增加是平行的，如荧光显微镜所见。在微管和中间细丝模式中只观察到微小的变化。而对照细胞含有很少的抗纤维连接蛋白反应物质，大量的这种物质出现在NaBT治疗。在暴露于NaBT的细胞中，纤维连接蛋白的数量增加了100倍。观察到的变化对应于通常与恶性表型相关的特性的抑制。
Sodium butyrate (NaBT) induces differentiation in several transformed cell lines. The present paper describes the effects of NaBT on some transformation-associated parameters in PC-3, a human prostatic carcinoma cell line. NaBT produces a reversible inhibition of cell proliferation, but anchorage-independent growth is more sensitive than monolayer growth. Soft agarose colonies are reduced by over 50% at 0.1 mM, a concentration that hardly affects growth on solid substrata. Monolayer cells respond to NaBT by spreading and flattening, as demonstrated by a combined light and electron microscopic, morphometric technique. After 4 days'' exposure to 2 mM NaBT, the average cell covers an area of substratum that is approximately double that covered by control cells. The average cell volume, however, remains unchanged. This flattening is paralleled by an increase in the number of stress fibers, as seen by fluorescence microscopy. Only minor changes are observed in the microtubule and intermediate filament patterns. While control cells contain very little antifibronectin reactive material, substantial amounts of such material appear upon NaBT treatment. The amount of fibronectin increases up to 100-fold in cells exposed to NaBT. The changes observed correspond to a suppression of properties that are generally associated with the malignant phenotype.