Dissolvable microneedle fabrication using piezoelectric dispensing technology
Dissolvable microneedle fabrication using piezoelectric dispensing technology
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DOI：
10.1016/j.ijpharm.2015.12.052
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发表时间：
2016-03-16
影响因子：
5.8
通讯作者：
Crean, Abina M.
中科院分区：
文献类型：
--
作者：
Allen, Evin A.;O'Mahony, Conor;Crean, Abina M.
可溶性微针（DMN）贴剂是用于经皮注射疫苗的新型剂型。 DMN 通常是通过将液体制剂分配到微针形模具中来制造的。然后将模具内的液体制剂干燥以产生可溶解的负载疫苗的微针。分配过程的精度对于控制加载到每个可溶解微针结构中的制剂体积至关重要。所采用的分配过程必须保持疫苗的完整性。分配配方对模具表面的润湿也是制造尖头 DMN 的一个重要考虑因素。尖头 DMN 对于易于经皮给药至关重要。在本文中，我们展示了压电分配系统将皮升制剂体积分配到 PDMS 模具中的能力，从而能够制造双层 DMN。描述了配方成分（海藻糖和聚乙烯醇（PVA）含量）和压电驱动参数（电压、频率和背压）对液滴形成的影响。研究了分配后季节性流感疫苗的生物完整性，并维持 30 V 的电压设置，但在更高的设置（50 和 80 V）下会受到破坏。结果证明了压电分配技术精确制造双层 DMN 的能力。他们还强调了确定配方和驱动参数以确保受控液滴配方和疫苗稳定性的重要性。 (C) 2015 Elsevier B.V. 保留所有权利。
Dissolvable microneedle (DMN) patches are novel dosage forms for the percutaneous delivery of vaccines. DMN are routinely fabricated by dispensing liquid formulations into microneedle-shaped moulds. The liquid formulation within the mould is then dried to create dissolvable vaccine-loaded microneedles. The precision of the dispensing process is critical to the control of formulation volume loaded into each dissolvable microneedle structure. The dispensing process employed must maintain vaccine integrity. Wetting of mould surfaces by the dispensed formulation is also an important consideration for the fabrication of sharp-tipped DMN. Sharp-tipped DMN are essential for ease of percutaneous administration.In this paper, we demonstrate the ability of a piezoelectric dispensing system to dispense picolitre formulation volumes into PDMS moulds enabling the fabrication of bilayer DMN. The influence of formulation components (trehalose and polyvinyl alcohol (PVA) content) and piezoelectric actuation parameters (voltage, frequency and back pressure) on drop formation is described. The biological integrity of a seasonal influenza vaccine following dispensing was investigated and maintained voltage settings of 30 V but undermined at higher settings, 50 and 80 V. The results demonstrate the capability of piezoelectric dispensing technology to precisely fabricate bilayer DMN. They also highlight the importance of identifying formulation and actuation parameters to ensure controlled droplet formulation and vaccine stabilisation. (C) 2015 Elsevier B.V. All rights reserved.