Surface Electric Fields of Apatite Electret Promote Osteoblastic Responses
Surface Electric Fields of Apatite Electret Promote Osteoblastic Responses
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DOI：
10.4028/www.scientific.net/kem.529-530.357
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Miho Nakamura;A. Nagai;K. Yamashita
中科院分区：
文献类型：
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作者：
Miho Nakamura;A. Nagai;K. Yamashita
生物材料基底上的成骨细胞行为被认为在骨传导过程中发挥着重要作用。本研究的目的是评估在电极化羟基磷灰石（HA）上培养的成骨细胞的体外行为，该成骨细胞具有增强的骨粘合能力。将来自小鼠骨髓的成骨细胞接种到极化的HA上并研究其增殖和分化。根据MTT测定，极化对成骨前体细胞的增殖有影响。加速是 N-HA 和 P-HA 汇合的早期成就。 ALP和AR-S染色结果定量分析，HA表面诱导的电荷加速了成骨前体细胞向成熟成骨细胞的分化。
The osteoblast behaviors on the biomaterial substrates are recognized to play a fundamental role in osteoconduction process. The purpose of this study was to evaluate the in vitro behaviors of osteoblasts cultured on electrically polarized hydroxyapatite (HA), having the enhanced osteobonding abilities. Osteoblasts derived from mouse bone marrow were seeded onto the polarized HA and investigated the proliferation and differentiation. The polarization had effects on the proliferation of osteoblast precursor cells based on the MTT assay. The acceleration was emerged as the early achievement to the confluence on the N-HA and P-HA. The quantitative analysis of the results of ALP and AR-S staining, the charges induced on the HA surface accelerated the differentiation from the osteoblast precursor cells to mature osteoblasts.