Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells
Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells
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DOI：
10.1016/j.ceca.2009.03.014
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发表时间：
2009-05-01
期刊：
影响因子：
4
通讯作者：
Zoratti, Mario
中科院分区：
文献类型：
--
作者：
De Marchi, Umberto;Sassi, Nicola;Zoratti, Mario
从人结肠癌116细胞中分离的膜片钳有丝分裂体允许识别和表征的中间电导钙激活的K+选择性通道K（Ca）3.1，以前只在质膜的各种细胞类型的研究。它的身份已确定其生物物理和药理学特性。它在线粒体内膜中的定位通过亚细胞组分的蛋白质印迹、通过记录其在线粒体中的活性（由线粒体靶向荧光蛋白发出荧光）以及被认为是内膜标记物的通道的共存来指示。线粒体基质[Ca 2 +]的适度增加将导致mtK（Ca）3.1开放，从而将内膜K+渗透性和跨膜电位与Ca 2+信号传导联系起来。(C)2009爱思唯尔有限公司版权所有。
Patch-clamping mitoplasts isolated from human colon carcinoma 116 cells has allowed the identification and characterization of the intermediate conductance Ca2+-activated K+-selective channel K(Ca)3.1, previously studied only in the plasma membrane of various cell types. Its identity has been established by its biophysical and pharmacological properties. Its localisation in the inner membrane of mitochondria is indicated by Western blots of subcellular fractions, by recording of its activity in mitochondria made fluorescent by a mitochondria-targeted fluorescent protein and by the co-presence of channels considered to be markers of the inner membrane. Moderate increases of mitochondrial matrix [Ca2+] will cause mtK(Ca)3.1 opening, thus linking inner membrane K+ permeability and transmembrane potential to Ca2+ signalling. (C) 2009 Elsevier Ltd. All rights reserved.