Functional Analysis of a Novel Immortalized Murine Microglia Cell Line in 3D Spheroid Model.
Functional Analysis of a Novel Immortalized Murine Microglia Cell Line in 3D Spheroid Model.
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3D球体模型中新型永生鼠小胶质细胞系的功能分析。
DOI：
10.1007/s11064-023-03952-6
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发表时间：
2023-09
影响因子：
4.4
通讯作者：
Wang, Chenran
中科院分区：
文献类型：
--
作者：
Angst, Gabrielle;Tang, Xin;Wang, Chenran
小胶质细胞是中枢神经系统的居住区免疫细胞，对脑发育和脑内稳态以及炎症性脑病的进展起着至关重要的作用。为了研究小胶质细胞的生理和病理功能，最广泛使用的模型之一是新生啮齿动物小胶质细胞的原代培养。然而，小胶质细胞的原代培养耗时长，需要大量的动物。在我们的小胶质细胞培养中，我们发现了一种自发永生化的小胶质细胞株，它在没有任何已知基因干预的情况下继续分裂。我们证实了这些细胞在不间断的30代中永生化，我们将它们命名为永生化小胶质样细胞-1细胞(IMG-1)。体外培养的IMG-1细胞保持小胶质细胞形态，表达巨噬细胞/小胶质细胞特异性蛋白CD11b、CD68、P2RY12和IBA1。IMG-1细胞对内毒素和多肌苷：多胞苷的炎症刺激有反应，触发IL-1-β、IL-6、肿瘤坏死因子-α和干扰素的基因/蛋白水平升高。脂多糖和pIpC处理的IMG-1细胞也显著增加其脂滴(LDs)的积累。我们还使用永生化的神经前体细胞和IMG-1细胞以确定的百分比建立了一个3D球体模型来研究神经炎症。IMG-1细胞均匀分布于球体中，它们调节3D球体中神经前体细胞因子的基础mRNA水平。IL-6和IL-1-β在球体中的表达增加，从而刺激IMG-1细胞对内毒素的反应。综上所述，本研究表明IMG-1的可靠性，可以方便地用于研究小胶质细胞的生理和病理功能。
Microglia are the residential immune cells of central nervous system and they are crucial for brain development and homeostasis, as well as the progression of inflammatory brain diseases. To study microglia’s physiological and pathological functions, one of the most widely used models is primary microglia culture from neonatal rodents. However, primary microglia culture is time consuming and needs a great number of animals. In our microglia culture, we found a strain of spontaneously immortalized microglia that continued to divide without any known genetic intervention. We confirmed the immortalization of these cells for uninterrupted thirty passages and we named them as immortalized microglia like-1 cells (iMG-1). The iMG-1 cells kept their microglia morphology, and they expressed macrophage/microglia-specific proteins of CD11b, CD68, P2RY12, and IBA1 in vitro. iMG-1 cells were responsive to inflammatory stimulations with lipopolysaccharide (LPS) and Polyinosinic:polycytidylic acid (pIpC), triggering increased mRNA/protein levels of IL1-β, IL-6, TNF-α, and interferons. LPS and pIpC treated iMG-1 cells also significantly increased their accumulation of lipid droplets (LDs). We also generated a 3D spheroid model using immortalized neural progenitor cells and iMG-1 cells with defined percentages to study neuroinflammation. The iMG-1 cells distributed evenly in spheroids, and they regulated the basal mRNA levels of cytokines of neural progenitors in 3D spheroid. iMG-1 cells were responsive to LPS by increased expression of IL-6 and IL1-β in spheroids. Together, this study indicated the reliability of iMG-1 which could be readily available to study the physiological and pathological functions of microglia.
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影响因子：
3.7
作者：
Hines DJ;Choi HB;Hines RM;Phillips AG;MacVicar BA
通讯作者：
MacVicar BA
影响因子：
4.2
作者：
Bachstetter, Adam D.;Morganti, Josh M.;Jernberg, Jennifer;Schlunk, Andrea;Mitchell, Staten H.;Brewster, Kaelin W.;Hudson, Charles E.;Cole, Michael J.;Harrison, Jeffrey K.;Bickford, Paula C.;Gemma, Carmelina
通讯作者：
Gemma, Carmelina
影响因子：
5.6
作者：
Cheng Z;Zhu W;Cao K;Wu F;Li J;Wang G;Li H;Lu M;Ren Y;He X
通讯作者：
He X
DOI：
10.1016/j.bbi.2015.11.001
发表时间：
2016-07
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
De Lucia C;Rinchon A;Olmos-Alonso A;Riecken K;Fehse B;Boche D;Perry VH;Gomez-Nicola D
通讯作者：
Gomez-Nicola D
影响因子：
6.5
作者：
Hall, Zoe;Wilson, Catherine H.;Griffin, Julian L.
通讯作者：
Griffin, Julian L.