[Redistribution of epidermal stem cells in wound edge in the process of re-epithelialization].
[Redistribution of epidermal stem cells in wound edge in the process of re-epithelialization].
复制标题
再上皮化过程中创面干细胞在创面的重新分布[J].
作者：
Jian;Xiao;Z. Sheng;T. Sun
目的
研究表皮干细胞在创面再生组织中的分布，阐明表皮干细胞在创面修复中的作用。
方法
在20只雄性Wistar大鼠背部两侧制作80个圆形全层伤口（每只动物4个伤口）。将80例创面随机分为两组：A组（磺胺嘧啶银锌霜治疗组，n = 40）和B组（未治疗组，n = 40）。肉眼观察创面感染及愈合过程。分别于伤后3、7、14和21天处死5只小鼠。从每只小鼠收集两个具有周围正常皮肤组织的伤口。常规组织学检查HE染色。应用免疫组化SP法检测表皮干细胞的标志物β 1整合素和角蛋白19（K19）在表皮干细胞中的分布。
结果
A组创面愈合率为80%（32/40），B组创面愈合率为60%（24/40）。在愈合过程中的任何时间点，在这两组的所有伤口的肉芽组织中均未发现β（1）整合素和K19阳性细胞。但在创面边缘表皮棘层和颗粒层中，有少量与基底层表皮干细胞无解剖关系的β 1整合素和K19阳性细胞散在分布。越靠近伤口，β（1）整联蛋白和角蛋白19阳性细胞越集中。随着创面的缩小，其数量沿着增加，直至创面完全愈合。上皮化后β 1整合素和角蛋白19阳性细胞逐渐减少。感染创面中β 1整合素和角蛋白19阳性细胞数明显低于未感染创面。
结论
表皮干细胞参与了创伤愈合过程。表皮干细胞在创面边缘的重新分布似乎促进了肉芽创面的再上皮化。
OBJECTIVE
To study the redistribution of epidermal stem cells in regenerating wound tissues, and to elucidate the role of epidermal stem cells during wound repair.
METHODS
80 circular full-thickness wounds were produced on both sides of the back in 20 male Wistar rats (4 wounds in each animal). Then the 80 wounds were randomly divided into 2 groups: group A (treated with sulfadiazine silver- sulfadiazine zinc cream, n = 40) and group B (without any treatment, n = 40). The infection and healing process of the wounds were observed with naked eyes. Five mice were killed one time 3, 7, 14, and 21 days after the wounding. Two wounds with surrounding normal skin tissues were collected from each mouse. Routine histological examination was conducted with HE staining. Beta(1) integrin and keratin19 (K19), markers of epidermal stem cells, were employed to determine the distribution of epidermal stem cells with streptavidin-peroxidase (SP) immunohistochemical method.
RESULTS
The healing rate of wounds was 80% (32/40) in group A, and 60% (24/40) in group B. No beta(1) integrin and K19 positive cells was found in the granulation tissue of all wounds in these 2 groups at any time point during the healing process. However, a few beta1 integrin and K19 positive cells, bearing no anatomic relation with the epidermal stem cells in the basal layer, were found scattering in the stratum spinosum and stratum granulosum of the epidermis on the wound edges. The closer to the wound the more concentrated the beta(1) integrin and keratin19 positive cells. Their numbers increased gradually along with the shrinking of the wound surface until the wound completely healed. The numbers of the beta(1) integrin and keratin19 positive cells decreased gradually after epithelialization. The numbers of the beta(1) integrin and keratin19 positive cells were obviously lower in the infected wounds than in the uninfected wounds.
CONCLUSION
Epidermal stem cells participated in the healing of wound. The function of redistribution of epidermal stem cells in wound edges seems to promote the re-epithelialization of granulating wounds.