Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses
Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses
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DOI：
10.1016/j.vetmic.2017.03.035
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发表时间：
2017-05-01
影响因子：
3.3
通讯作者：
Weng, Changjiang
中科院分区：
文献类型：
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作者：
Li, Jiangnan;Wang, Shengnan;Weng, Changjiang
高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）感染常使猪发生继发性细菌感染，引起强烈的炎症反应。然而，继发性细菌感染是否协同HP-PRRSV感染并增强炎症反应尚未完全了解。在这里，我们的特点是HP-PRRSV感染介导的继发性细菌感染和强大的炎症反应。HP-PRRSV感染可诱导仔猪血清中细胞因子（IL-1 β、IL-18、IL-6和TNF-α）水平升高，并导致猪鼻支原体、副猪嗜血杆菌和大肠杆菌等11种细菌的载量增加。同时感染HP-PRRSV和H. parasuis模型的研究表明，感染HP-PRRSV或H.独自一人此外，我们还发现H.在HP-PRRSV感染的PAM中，副猪RNA在强烈的炎症反应增强中起重要作用。综上所述，我们的研究结果表明，细菌RNA转染增强HP-PRRSV介导的炎症反应在HP-PRRSV和H。本研究为全面认识HP-PRRSV与细菌共感染介导的病理学提供了重要线索。
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection often predisposes pigs to secondary bacterial infection, which induces robust inflammatory responses. However, whether the secondary bacterial infection synergizes HP-PRRSV infection and enhances inflammatory responses is not fully understood. Here, we characterized HP-PRRSV infection-mediated secondary bacterial infection and robust inflammatory responses. HP-PRRSV infection induced higher levels of cytokines (IL-1 beta, IL-18, IL-6 and TNF-alpha) in the sera in piglets and bacterial loads of 11 bacterial species in the lung were increased after HP-PRRSV infection, including Mycoplasma hyorhinis, Haemophilus parasuis and Escherichia coli. Concurrent infection with HP-PRRSV and H. parasuis model showed that inflammatory cytokines expression and secretion in porcine alveolar macrophages (PAMs) were increased in comparison with PAMs infected with HP-PRRSV or H. parasuis alone. Additionally, we found that H. parasuis RNA plays an important role in the robust inflammatory response enhancement in HP-PRRSV-infected PAMs. Taken together, our findings suggest that bacterial RNA transfection enhanced HP-PRRSV-mediated inflammatory responses in HP-PRRSV and H. parasuis (HPS) concurrent infection, which provides an important clue for comprehensive understanding of HP-PRRSV and bacterial coinfectionmediated pathology.