Effect of Laggera alata on hepatocyte damage induced by carbon tetrachloride in vitro and in vivo
Effect of Laggera alata on hepatocyte damage induced by carbon tetrachloride in vitro and in vivo
复制标题
阿拉塔对四氯化碳体内外肝细胞损伤的影响
DOI：
10.1016/j.jep.2009.08.030
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发表时间：
2009-10-29
影响因子：
5.4
通讯作者：
Zhao, Yu
中科院分区：
文献类型：
--
作者：
Wu, Yi-Hang;Zhang, Xiao-Meng;Zhao, Yu
民族药理相关性：Laggera alata。作为一种传统的中草药，在民间被广泛用于改善包括肝炎在内的一些与炎症相关的疾病。本研究的目的：为进一步验证狼毒总酚的抗炎活性，进一步验证其对肝炎的显著疗效，考察其对肝脏的保护作用。材料与方法：制备雷公藤总酚并对其主要成分进行定量分析。采用CCl4诱导的原代培养大鼠肝细胞损伤模型和CCl4诱导的急性和慢性肝损伤模型，研究Tpla对肝细胞的保护作用。结果：Tpla可显著降低肝细胞天冬氨酸转氨酶(AST)和丙氨酸氨基转移酶(ALT)的漏出量，提高细胞存活率。能显著降低小鼠血清AST、ALT水平，降低大鼠血清AST、ALT、总蛋白、白蛋白、唾液酸水平，降低大鼠肝脏羟脯氨酸水平。同时，TPLA能明显改善CCl4所致小鼠/大鼠的严重肝损伤。结论：本实验验证了TPLA体内外的保肝作用，并阐明了其具有保肝作用的活性成分二咖啡酰奎宁酸。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: Laggera alata. as a traditional Chinese herbal medicine, has been widely used to ameliorate some ailments associated with inflammation including hepatitis in folk. Aim of the study: Based oil anti-inflammatory activity of total phenolics from Laggera alata (TPLA), to further validate the remarkable curative effect Laggera alata in hepatitis, hepato protective effect of TPLA was examined.Materials and methods:TPLA was prepared and its principle components were quantificationally analyzed. The hepatoprotective effects of TPLA were studied using a CCl4-induced injury model in primary cultured neonatal rat hepatocytes, and a CCl4-induced acute and chronic damage model in vivo.Results: TPLA significantly reduced cellular leakage of hepatocyte aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and improved cell viability in vitro. TPLA markedly decreased the serum AST and ALT levels of the mice, the levels of AST, ALT, total protein, albumin, and sialic acid in rat serum, and the hydroxyproline level in rat liver. Meanwhile, severe hepatic lesions induced by CCl4 in mice/rats were remarkably improved by the administration of TPLA.Conclusions: This investigation verifies the hepatoprotective effect of TPLA in vitro/in vivo and clarifies its active components dicaffeoylquinic acids responsible for hepatoprotective potential. (C) 2009 Elsevier Ireland Ltd. All rights reserved.