Subgingival distribution of Campylobacter rectus and Tannerella forsythensis in healthy children with primary dentition
Subgingival distribution of Campylobacter rectus and Tannerella forsythensis in healthy children with primary dentition
复制标题
DOI：
10.1016/j.archoralbio.2005.05.004
复制
发表时间：
2006-01-01
影响因子：
3
通讯作者：
Kozai, K
中科院分区：
文献类型：
--
作者：
Hayashi, F;Okada, M;Kozai, K
目的：重要的是要知道应该检测多少儿童的龈下菌斑样本，以确定是否感染了牙周病原体。如果只对有限数量的牙龈部位进行采样，来自几个地点的菌斑样本可能无法检测出一些重要的细菌。本研究的目的是评估在同一儿童的大量龈下部位进行牙周病原体检测的情况，以确定所需的样本数量。方法：选择10名4~6岁的乳牙列完整儿童。每颗萌出牙近中颊侧面的菌斑样本首先轻轻插入无菌纸尖，取10个S样本，提取菌斑基因组DNA，进行聚合酶链式反应。以物种特异性16S核糖体RNA序列的引物为目标序列。结果：所有受试者均检出直肠弯曲菌和连翘杆菌阳性，其中直肠弯曲菌阳性点数平均为17.6+/-2.4(12~20个)，连翘阳性点数平均为9.3+/-5.0(1~19个)。直肠弯曲菌平均阳性点数为1.7+/-0.8，连翘阳性点数为6.5+/-4.9，置信度为95%。结论：儿童直肠弯曲菌有2个或2个以上的随机点检出，连翘有7个或7个以上的随机点检出，检出概率为95%。(C)2005爱思唯尔有限公司。保留所有权利。
Objective: It is important to know how many subgingival plaque samples should be assayed from a child to ascertain infection with a periodontal pathogen. Plaque samples from several sites may fait to detect some important bacteria if only a limited number of gingival sites are sampled. The purpose of this study was to evaluate the detection of periodontal pathogens in a large number of subgingival sites in the same children in order to determine the number of samples necessary.Methods: Ten children, aged 4-6 years, with complete primary dentition were enrolled in this study. Plaque samples from the mesio-buccal aspect of each erupted tooth were first collected by gently inserting a sterile paper point for 10 s. Purified genomic DNA from at[ plaque samples was prepared for potymerase chain reaction. The primers for species-specific 16S ribosomal RNA sequence were selected as the target sequence. Standard strains of Campylobacter rectus and Tannerella forsythensis (formerly Bacteroides forsythus) were used as control strains.Results: All subjects were found positive for C. rectus and T forsythensis with the mean of positive sites at 17.6 +/- 2.4 (range: 12-20 sites) for C. rectus and 9.3 +/- 5.0 (range: 1-19) for T forsythensis. The mean number of positive sites was 1.7 +/- 0.8 for C. rectus and 6.5 +/- 4.9 for T forsythensis, with a confidence ratio of 95%.Conclusions: We concluded that two or more random sites for C. rectus and seven or more random sites for T forsythensis from children to detect those bacteria at 95% probability. (c) 2005 Elsevier Ltd. All rights reserved.