Characterization of a novel piscidin-like antimicrobial peptide from Pseudosciaena crocea and its immune response to Cryptocaryon irritans
Characterization of a novel piscidin-like antimicrobial peptide from Pseudosciaena crocea and its immune response to Cryptocaryon irritans
复制标题
大黄鱼新型鱼腥素样抗菌肽的表征及其对白点虫刺激的免疫反应
DOI：
10.1016/j.fsi.2013.05.007
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发表时间：
2013-08-01
影响因子：
4.7
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Niu, Su-Fang;Jin, Yuan;Wang, Jun
鱼杀菌素是鱼类天然（非特异性）免疫系统的重要组成部分，具有广谱的抗菌和抗寄生虫活性。本研究报道了一种新的大黄鱼抗菌阳离子肽。虽然该肽具有与已报道的鱼类piscidins相同的基因组（3个外显子和2个内含子）和前肽（信号肽、成熟肽和前结构域）结构、保守的信号肽（22个氨基酸）和共有基序I-X5-H-X4-I-H，但Pc-pis与其他已知piscidins的保守性较低，这明显体现在肽的氨基酸组成上。Pc-pis富含甘氨酸残基（27.3%），这破坏了肽的两亲性结构。实时荧光定量PCR结果表明，Pc-pis是一种典型的鳃表达肽。序列分析、结构特征和组织分布表明，Pc-pis与piscidins家族具有遗传相关性，可能是一种新的piscidin类抗菌肽。定量PCR分析表明，Pc-pis在刺激隐核虫感染过程中和感染后，在脾脏、头肾、肝脏、肠道、皮肤和鳃等组织中的表达均受到调控。Pc-pis在抗刺激梭菌和继发性细菌感染的免疫防御中的作用。合成的Pc-pis对细菌、真菌和C.在寄生阶段的irritans。这些结果首次证明了piscidin对海洋鱼类体外寄生虫C. Pc-pis可能是大黄鱼天然免疫系统的重要组成部分。因此，这些数据提供了新的见解P. crocea先天免疫对外部原生动物寄生虫和微生物感染，并促进Pc-pis作为治疗剂对病原体入侵的评估。(C)2013爱思唯尔有限公司保留所有权利。
Piscidins, important components of the innate (nonspecific) immunity system in fish, have potent, broad-spectrum antimicrobial and antiparasitic activities. In this study, we reported a novel antimicrobial cationic peptide from Pseudosciaena crocea. Although this peptide exhibited a genomic (3 exons and 2 introns) and propeptide (signal peptide, mature peptide and prodomain) organization, conserved signal peptide (22 amino acids) and consensus motif I-X5-H-X4-I-H identical to the reported fish piscidins, Pc-pis showed a relatively low overall conservation with other known piscidins, which was obviously embodied in the amino acid composition of the peptide. Pc-pis is strikingly rich in glycine residues (27.3%), which disrupted the amphipathic structure of the peptide. Relative quantitative real-time PCR revealed that Pc-pis is a typically gill-expressed peptide. The sequence analysis, structural features and tissue distribution suggested that Pc-pis was genetically related to the piscidins family and might be a novel piscidin-like antimicrobial peptide. Quantitative PCR analysis revealed that the expression of Pc-pis in the spleen, head-kidney, liver, intestine, skin and gill could be regulated during Cryptocaryon irritans infection and post C. irritans falling off, implicating a role for Pc-pis in immune defense against C irritans and secondary bacterial infections. Synthetic Pc-pis exhibited broad-spectrum activity against bacteria, fungi and C. irritans in parasitic stages. These results provided the first evidence of piscidins antiparasitic activity against marine fish ectoparasites C. irritants trophonts and further indicated that Pc-pis might be an important component of the P. crocea innate immune system against C irritans and secondary bacterial infections. Thus, these data provided new insights into P. crocea innate immunity against external protozoan parasite and microbial infections and facilitate the evaluation of Pc-pis as a therapeutic agent against pathogen invasion. (C) 2013 Elsevier Ltd. All rights reserved.