Heterogeneity in telomere length of human chromosomes
Heterogeneity in telomere length of human chromosomes
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DOI：
10.1093/hmg/5.5.685
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发表时间：
1996-05-01
影响因子：
3.5
通讯作者：
Tanke, HJ
中科院分区：
文献类型：
--
作者：
Lansdorp, PM;Verwoerd, NP;Tanke, HJ
脊椎动物染色体终止于不同数量的 T(2)AG(3) 核苷酸重复序列，为了研究单个染色体上的端粒重复序列，我们使用标记的 (C(3)TA(2))(3) 肽核酸和数字成像显微镜开发了新颖的定量荧光原位杂交程序。培养的人类造血细胞中期染色体的端粒荧光强度值随着细胞的复制历史而减少，在中期内变化高达六倍，并且姐妹染色单体端粒之间相似。令人惊讶的是，正常成人骨髓中期内的端粒荧光强度值并未显示出正态分布，这表明在正常造血过程中需要和/或维持每个端粒的最小重复次数。
Vertebrate chromosomes terminate in variable numbers of T(2)AG(3) nucleotide repeats, In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C(3)TA(2))(3) peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis.