A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice.
A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice.
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DOI：
10.1038/bjc.1995.163
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发表时间：
1995-04
影响因子：
8.8
通讯作者：
Sonoo, H
中科院分区：
文献类型：
--
作者：
Kurebayashi, J;Kurosumi, M;Sonoo, H
我们最近建立了一种新的人类乳腺细胞系，命名为 KPL-1，该细胞系源自乳腺癌患者的恶性积液。该细胞系具有高度致瘤性，并且在雌性裸鼠中生长迅速。细胞遗传学分析表明其起源于人类，并揭示了超三倍体染色体众数。电子显微镜检查表明KPL-1细胞是上皮来源的。免疫组织化学研究表明，这些细胞表达细胞角蛋白、癌胚抗原和 CA 15-3。它们还拥有大量雌激素受体，但没有孕激素受体。有趣的是，KPL-1 细胞似乎在体外独立生长雌激素。未检测到 c-erbB-2、c-myc、H-ras 和 N-ras 基因的扩增。 KPL-1细胞分泌大量的组织多肽抗原（TPA）。尽管 CA 15-3 的分泌在所有细胞生长阶段似乎都是恒定的，但 TPA 分泌在指数生长期期间增加，并在平台期期间减少。血清TPA水平与裸鼠移植的KPL-1肿瘤体积显着相关。这些数据表明，该 KPL-1 细胞系可用于研究体内和体外的雌激素非依赖性生长和肿瘤相关抗原的动力学。
We recently established a new human breast cell line, designated KPL-1, which was derived from the malignant effusion of a patient with breast cancer. This cell line is highly tumorigenic and grows rapidly in female nude mice. Cytogenetic analysis indicated its human origin and revealed a hypertriploid modal number of chromosomes. Electron microscopic examination suggested that the KPL-1 cells are of epithelial origin. Immunohistochemical studies revealed that the cells express cytokeratin, carcinoembryonic antigen and CA 15-3. They also possess a large number of oestrogen receptors but not progesterone receptors. Interestingly, KPL-1 cells seem to grow oestrogen independently in vitro. No amplification of c-erbB-2, c-myc, H-ras and N-ras genes was detected. KPL-1 cells secrete a large amount of tissue polypeptide antigen (TPA). Although the secretion of CA 15-3 seemed to be constant throughout all cell growth phases, TPA secretion increased during the exponential growth phase and decreased during the plateau phase. Serum TPA levels significantly correlated with the volume of KPL-1 tumours transplanted into nude mice. These data suggest that this KPL-1 cell line may be useful for studying oestrogen-independent growth and the kinetics of tumour-associated antigens in vivo as well as in vitro.