INTEGRATIVE RECOMBINATION OF BACTERIOPHAGE-LAMBDA - EXTENT OF THE DNA-SEQUENCE INVOLVED IN ATTACHMENT SITE FUNCTION
INTEGRATIVE RECOMBINATION OF BACTERIOPHAGE-LAMBDA - EXTENT OF THE DNA-SEQUENCE INVOLVED IN ATTACHMENT SITE FUNCTION
复制标题
DOI：
10.1073/pnas.77.6.3220
复制
发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
MIZUUCHI, K
中科院分区：
文献类型：
--
作者：
MIZUUCHI, M;MIZUUCHI, K
噬菌体.lambda附着位点所需的最小DNA序列范围。研究了在整合重组中的功能。携带噬菌体λ的噬菌体附着位点(attP)的DNA片段。经过修剪、重新克隆并测试重组能力。为了与细菌附着位点 (attB) 重组，噬菌体附着位点必须保留其原始序列的约 250 个碱基对。在左侧，基本序列从 15 碱基对共同核心序列的中心延伸超过 106 个碱基对，但不超过 152 个碱基对。在右侧，所需序列从核心中心延伸超过 68 个碱基对，但不超过 99 个碱基对。丢失上述部分序列的修剪位点不能充当噬菌体附着位点。根据存在序列的哪一部分，这样的位点仍然可以在通常需要前噬菌体附着位点或细菌附着位点之一的反应中起作用。细菌[大肠杆菌]附着位点的基本序列仅由噬菌体和细菌附着位点共有的序列组成。
The minimum extent of DNA sequence required for the attachment site of bacteriophage .lambda. to function in integrative recombination is investigated. A DNA fragment carrying the phage attachment site (attP) of bacteriophage .lambda. was trimmed, recloned and tested for recombination proficiency. To recombine with the bacterial attachment site (attB), the phage attachment site must retain about 250 base pairs of its original sequence. On the left side, the essential sequence extends beyond 106 base pairs from the center of the 15-base-pair common core sequence but not beyond 152 base pairs. On the right side the required sequence extends beyond 68 base pairs but not beyond 99 base pairs from the center of the core. A trimmed site that has lost part of the sequence mentioned above cannot function as the phage attachment site. Depending on which part of the sequence is present, such a site can still act in reactions normally requiring one of the prophage attachment sites or the bacterial attachment site. The essential sequence of the bacterial [Escherichia coli] attachment site consists only of the sequence common to the phage and bacterial attachment sites.