Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (Solanum tuberosum).
Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (Solanum tuberosum).
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发表时间：
2014-04
影响因子：
1.4
通讯作者：
Imtiaj Hasan;Y. Ozeki;S. R. Kabir
中科院分区：
文献类型：
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作者：
Imtiaj Hasan;Y. Ozeki;S. R. Kabir
从孟加拉国马铃薯品种‘Deshi’中分离纯化了一种新的几丁质结合凝集素。通过使用几丁质柱的阴离子交换和亲和色谱法。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示凝集素的分子量为20，000道尔顿。该分子量几乎是来自其他马铃薯的几丁质结合凝集素分子量的一半。该凝集素对革兰氏阳性（单核细胞增生李斯特菌）和革兰氏阴性（大肠杆菌、沙门氏菌和鲍氏志贺菌）致病菌均具有杀菌和生长抑制活性。它还显示出对根霉属的抗真菌活性，青霉属和尼日尔曲霉。由细菌铜绿假单胞菌产生的生物膜在施用凝集素后24小时内剂量依赖性地减少5-20%，这归因于凝集素对GlcNAc聚合物具有亲和力的聚糖结合性质。这是第一次观察到任何马铃薯凝集素都可以防止铜绿假单胞菌形成生物膜，因此可能在临床微生物学和生物医学科学中有可能的应用。
A new chitin-binding lectin was purified from a Bangladeshi cultivar 'Deshi' of potato (Solanum tuberosum L.) through anion-exchange and affinity chromatographies using a chitin column. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the molecular mass of the lectin as 20,000 Daltons. This molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. The lectin showed both bactericidal and growth-inhibiting activities against Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli, Salmonella enteritidis and Shigella boydii) pathogenic bacteria. It also showed antifungal activity against Rhizopus spp., Penicillium spp. and Aspergillus niger. Biofilm produced by the bacterium Pseudomonas aeruginosa was dose-dependently reduced by 5-20% in 24 h after administration of the lectin, which was attributed to the glycan-binding property of the lectin having affinity to GlcNAc polymers. It was the first observation that any potato lectin prevented biofilm formation by P. aeruginosa and, therefore, could have possible applications in clinical microbiology and biomedical science.