Luteotropic and luteolytic responsiveness of ovine luteal cells in long-term culture.
Luteotropic and luteolytic responsiveness of ovine luteal cells in long-term culture.
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长期培养中绵羊黄体细胞的促黄体和黄体溶解反应。
DOI：
10.1095/biolreprod41.4.707
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发表时间：
1989
影响因子：
3.6
通讯作者：
Hoyer,PB
中科院分区：
文献类型：
--
作者：
Kong,W;Marion,SL;Hoyer,PB
在注射人绒毛膜促性腺激素(0d)后36h(第2天)采集绵羊黄体细胞，诱导排卵。细胞在含5%小牛血清的199培养液中维持(第2-12天)，每天更换。黄体生成素(L H)(10 ng/m l或100 ng/m l)在培养过程中的任何时间均不刺激孕酮的分泌(p>；0.05)。然而，在培养早期(基础刺激2.2倍，第5天，6天)或中期(1.7倍刺激：基础8，9天)培养早期(刺激强度是基础刺激的2.2倍；第5、6天)或中期(刺激强度是基础刺激的1.7倍：第8天、第9天)时，加入24小时脉冲的二丁酰腺苷3‘，5’-一磷酸(DBcAMP)可增强孕酮分泌(p<；0.05)，但如果提取血清24 h则不能。3-羟基-Δ-5-类固醇脱氢酶-Δ5，Δ-4-异构酶(3-β-HSD)染色阳性细胞百分率在培养12天(3-12天)恢复。继续与前列腺素F2α(PgF2α)孵育，可剂量依赖性地抑制孕酮的分泌。从这些培养中回收了数量减少的3个β-HSD阳性细胞。这些实验表明，在长期培养的绵羊黄体细胞中，促黄体素(DBcAMP)和促黄体生成素(PGF2)α的作用相同。这项研究提供的证据表明，这些培养物将有助于研究黄体功能的激素调节的发展。
Ovine luteal cells were collected and plated 36 h (Day 2) after injection of human chorionic gonadotropin (Day 0) to induce ovulation. Cells were maintained (Days 2–12) in Medium 199 containing 5% calf serum, which was replaced daily. Progesterone secretion was not stimulated (p > 0.05) by luteinizing hormone (LH, 10 ng/ml or 100 ng/ml) at any time during culture. However, it was enhanced (p < 0.05) with a 24-h pulse of dibutyryl adenosine 3′, 5′-monophosphate (dbcAMP) during early (2.2-fold stimulation over basal; Days 5,6) or mid- (1.7-fold stimulation over basal: Days 8,9) culture if the pulsing medium contained serum, but not if serum had been withdrawn for 24 h. Continuous exposure of cultures to dbcAMP (2 mM, Days 3–12) resulted in continuously stimulated (p < 0.05) progesterone secretion (range 1.8- to 4.1-fold stimulation). An increased (p < 0.05) percentage of cells staining positive for 3 β-hydroxy-Δ5-steroid dehydrogenase-Δ5, Δ4-isomerase (3β HSD) activity were recovered on Day 12 in cultures incubated (Days 3–12) with dbcAMP. Incubation of cultures continuously with prostaglandin F2α(PGF2α) produced dose-dependent inhibition (p < 0.05) of progesterone secretion. Reduced numbers of 3β HSD-positive cells were recovered from these incubations. These experiments demonstrate luteotropic (dbcAMP) as well as luteolytic (PGF2α) effects on ovine luteal cells in long-term culture. This study provides evidence that these cultures will be useful for investigating the development of hormonal regulation of luteal function.
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影响因子：
3.6
作者：
P. Hoyer;W. Kong;E. G. Crichton;L. Bevan;P. Krutzsch
通讯作者：
P. Hoyer;W. Kong;E. G. Crichton;L. Bevan;P. Krutzsch
人黄体细胞单层培养物的激素分泌。
DOI：
10.1210/jcem-53-4-890
发表时间：
1981
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Goldsmith,LT;Essig,M;Sarosi,P;Beck,P;Weiss,G
通讯作者：
Weiss,G
黄体生成素和二丁酰环AMP诱导组织培养中牛黄体细胞的形态转变和生长抑制。
影响因子：
4.8
作者：
D. Gospodarowicz;F. Gospodarowicz
通讯作者：
F. Gospodarowicz
钙是黄体细胞中黄体生成素敏感的腺苷酸环化酶的抑制剂。
影响因子：
4.8
作者：
Dorflinger,LJ;Albert,PJ;Williams,AT;Behrman,HR
通讯作者：
Behrman,HR
母羊整个动情周期和妊娠早期 LH 黄体受体的方法验证和定量。
影响因子：
3.6
作者：
M. Diekman;P. W. O'Callaghan;T. Nett;G. Niswender
通讯作者：
G. Niswender