Pax-5 protein expression in bladder cancer: a preliminary study that shows no correlation to grade, stage or clinical outcome
Pax-5 protein expression in bladder cancer: a preliminary study that shows no correlation to grade, stage or clinical outcome
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DOI：
10.1080/00313020802197871
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Stoehr, Robert
中科院分区：
文献类型：
--
作者：
Denzinger, Stefan;Burger, Maximilian;Stoehr, Robert
目的：Pax（配对盒）基因包括一个对细胞分化至关重要的基因家族，编码一组转录因子。最近，Pax-5 mRNA表达被认为是膀胱癌（BC）的预后标志物。然而，Pax-5在BC中的功能作用是值得怀疑的，因为在这些研究中没有确定蛋白质表达。因此，我们评估了Pax-5蛋白表达在一个连续的系列的BC.Methods：我们pathemically研究了Pax-5蛋白表达在100档案膀胱肿瘤和22个正常尿路上皮样本，使用组织芯片（TMA）技术和单克隆抗体对Pax-5。染色强度和阳性染色细胞的百分比进行了测定，并与肿瘤的组织病理学特征和临床随访data.Results：所有22例组织病理学正常的尿路上皮细胞Pax-5蛋白表达呈阴性。总体而言，100个肿瘤中有70个给出了可解释的结果。70例中仅7例（10%）细胞核Pax-5染色阳性，但与临床病理特征无明显相关性。有趣的是，我们可以观察到Pax-5阳性淋巴细胞位于肿瘤内或密切相邻的基础间质中的24 70例（34%）在我们的series.Conclusions：Pax-5蛋白表达是罕见的BC。与临床病理特征的相关性的缺乏表明Pax-5在BC中的次要功能作用。Pax-5阳性淋巴细胞在肿瘤附近的反应性浸润需要进一步的研究，评估生物学，免疫学和临床相关性。
Aims: Pax (paired box) genes comprise a gene family crucial for cell differentiation that encodes a set of transcription factors. Recently, Pax-5 mRNA expression was suggested as a prognostic marker in bladder cancer (BC). However, a functional role of Pax-5 in BC is questionable because the protein expression was not determined in these studies. Therefore, we evaluated Pax-5 protein expression in an unselected, consecutive series of BC.Methods: We immunohistochemically investigated Pax-5 protein expression in 100 archival bladder tumours and 22 normal urothelial samples using tissue microarray (TMA) technology and a monoclonal antibody against Pax-5. Staining intensity and percentage of positively stained cells were determined and correlated to histopathological characteristics of the tumours and clinical follow-up data.Results: All 22 samples of histopathologically normal urothelium were negative for Pax-5 protein expression. Overall, 70 of 100 tumours gave interpretable results. Only seven of 70 (10%) cases showed a positive nuclear Pax-5 staining but without significant correlation to clinicopathological characteristics. Interestingly, we could observe Pax-5 positive lymphocytes located within the tumour or closely adjacent in the underlying stroma in 24 of 70 (34%) cases in our series.Conclusions: Pax-5 protein expression is infrequent in BC. Absence of correlation to clinicopathological characteristics suggests a minor functional role of Pax-5 in BC. Pax-5 positive lymphocytes within reactive infiltrates adjacent to the tumour warrant further studies evaluating biological, immunological and clinical relevance.