Next generation sequencing based approaches to epigenomics
Next generation sequencing based approaches to epigenomics
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DOI：
10.1093/bfgp/elq035
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发表时间：
2010-12-01
影响因子：
4
通讯作者：
Marra, Marco A.
中科院分区：
文献类型：
--
作者：
Hirst, Martin;Marra, Marco A.
下一代测序将表观基因组研究带入了当前研究的前沿。大规模并行测序与创新的分子和计算技术相结合的力量，使研究人员能够以仅仅几年前还无法想象的分辨率来描绘表观基因组。随着早期概念验证研究的发表，该领域现在正在进入下一个阶段，方法标准化和严格的质量控制的重要性变得至关重要。在这篇综述中，我们将描述已经开发的使用下一代测序平台来描述表观基因组的方法。我们将从文库准备、测序平台和分析技术等方面对此进行讨论。
Next generation sequencing has brought epigenomic studies to the forefront of current research. The power of massively parallel sequencing coupled to innovative molecular and computational techniques has allowed researchers to profile the epigenome at resolutions that were unimaginable only a few years ago. With early proof of concept studies published, the field is now moving into the next phase where the importance of method standardization and rigorous quality control are becoming paramount. In this review we will describe methodologies that have been developed to profile the epigenome using next generation sequencing platforms. We will discuss these in terms of library preparation, sequence platforms and analysis techniques.