Determination of gene promoter activity in skeletal muscles in vivo.
Determination of gene promoter activity in skeletal muscles in vivo.
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体内骨骼肌基因启动子活性的测定。
DOI：
10.1007/978-1-61779-343-1_27
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Judge,AndrewR
中科院分区：
文献类型：
--
作者：
Senf,SarahM;Judge,AndrewR
The use of nonviral (plasmid DNA) gene delivery into skeletal muscle has increased significantly in recent years. The procedure is used to overexpress wild-type proteins, express mutant proteins, or knock down endogenous proteins. These manipulations can identify the role of a specific protein in muscle cell biology and physiology. The same procedure of plasmid DNA gene delivery can be used to introduce a gene promoter reporter construct. Such constructs contain a defined sequence of a gene promoter that regulates the expression of a “reporter.” This reporter is easily measured and reflects the in vivo transcriptional activity of the gene promoter sequence under study. The gene promoter can be mutated at known transcription factor-binding sites, truncated to identify specific regions of the gene promoter that are required for transcription, or introduced into skeletal muscle with an expression plasmid for a protein believed to regulate the gene’s transcription. Therefore, the use of such gene promoter reporters allows for an in-depth physiological assessment of the gene’s transcriptional regulation.
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体外和体内基因调控之间的不一致。
影响因子：
--
作者：
Kitsis,RN;Leinwand,LA
通讯作者：
Leinwand,LA
通过电穿孔在小鼠骨骼肌中进行基于质粒的基因转移。
影响因子：
--
作者：
J. Schertzer;G. Lynch
通讯作者：
G. Lynch
DOI：
10.1152/ajplung.00368.2009
发表时间：
2010-06-01
影响因子：
4.9
作者：
Bao, Shengying;Liu, Ming-Jie;Knoell, Daren L.
通讯作者：
Knoell, Daren L.
影响因子：
15.9
作者：
Acharyya, Swarnali;Villalta, S. Armando;Guttridge, Denis C.
通讯作者：
Guttridge, Denis C.
影响因子：
5.5
作者：
Senf, Sarah M.;Dodd, Stephen L.;Judge, Andrew R.
通讯作者：
Judge, Andrew R.