Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation.
Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation.
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DOI：
10.3390/genes9080413
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发表时间：
2018-08-15
期刊：
影响因子：
3.5
通讯作者：
Palmisano G
中科院分区：
文献类型：
--
作者：
Avila CC;Mule SN;Rosa-Fernandes L;Viner R;Barisón MJ;Costa-Martins AG;Oliveira GS;Teixeira MMG;Marinho CRF;Silber AM;Palmisano G
克氏锥虫（Trypanosoma cruzi）是南美锥虫病的病原体，其通过不同的生命阶段循环，其特征在于与增殖或分化状态相关的确定的分子特征。特别是T.克氏上鞭毛体是在进入稳定期之前定殖于锥虫属昆虫载体的肠的复制形式，所述稳定期对于分化成亚循环锥虫鞭毛体是至关重要的，所述亚循环锥虫鞭毛体是哺乳动物宿主的感染形式。从增殖指数阶段到静止稳定阶段的过渡是一个重要的步骤，概括了早期的分子事件的后生，了解这一过程开辟了新的可能性。在这项研究中，我们报告了定量鸟枪蛋白质组学分析的T。cruzi外鞭毛体在指数生长期和静止生长期的形态学变化。超过3000种蛋白质被检测和定量，突出了参与不同亚细胞区室的蛋白质的调节。核糖体蛋白在指数期上调，支持该生长期的较高复制率。自噬相关蛋白在静止生长期上调，表明的metacyclogenesis过程的开始。此外，这项研究报告了N-末端乙酰化蛋白在生长阶段过渡期间的调节，为这一过程增加了一个新的调节层。总之，这项研究报告了T。Cruzi从复制指数期过渡到稳定生长期，这是分化的准备期。
Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state. In particular, T. cruzi epimastigotes are the replicative forms that colonize the intestine of the Triatomine insect vector before entering the stationary phase that is crucial for differentiation into metacyclic trypomastigotes, which are the infective forms of mammalian hosts. The transition from proliferative exponential phase to quiescent stationary phase represents an important step that recapitulates the early molecular events of metacyclogenesis, opening new possibilities for understanding this process. In this study, we report a quantitative shotgun proteomic analysis of the T. cruzi epimastigote in the exponential and stationary growth phases. More than 3000 proteins were detected and quantified, highlighting the regulation of proteins involved in different subcellular compartments. Ribosomal proteins were upregulated in the exponential phase, supporting the higher replication rate of this growth phase. Autophagy-related proteins were upregulated in the stationary growth phase, indicating the onset of the metacyclogenesis process. Moreover, this study reports the regulation of N-terminally acetylated proteins during growth phase transitioning, adding a new layer of regulation to this process. Taken together, this study reports a proteome-wide rewiring during T. cruzi transit from the replicative exponential phase to the stationary growth phase, which is the preparatory phase for differentiation.
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影响因子：
3.2
作者：
Caballero ZC;Costa-Martins AG;Ferreira RC;P Alves JM;Serrano MG;Camargo EP;Buck GA;Minoprio P;G Teixeira MM
通讯作者：
G Teixeira MM
影响因子：
3.2
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作者：
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通讯作者：
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影响因子：
1.5
作者：
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通讯作者：
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影响因子：
3.4
作者：
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