Calcium-tension relationships of muscle fibers from patients with periodic paralysis.
Calcium-tension relationships of muscle fibers from patients with periodic paralysis.
复制标题
周期性麻痹患者肌纤维的钙-张力关系。
DOI：
10.1002/mus.880140907
复制
发表时间：
1991
期刊：
影响因子：
3.4
通讯作者：
Ruff,RL
中科院分区：
文献类型：
--
作者：
Ruff,RL
研究了1例26岁高钾型周期性麻痹男性和1例23岁低钾型周期性麻痹男性的腓肠肌外侧活检。这两名患者都来自家族，其年长的亲属患有与周期性麻痹相关的空泡性肌病。对肌纤维进行化学剥皮，并使用低顺应性应变计对单个纤维进行研究。在钙浓度为10 - 8 mol/L至2.5 × 10 - 5 mol/L的浴中研究了纤维产生的张力。快、慢颤肌纤维的Ca-张力关系和最大张力（归一化为纤维横截面积）与5名正常受试者的纤维中发现的张力关系和最大张力无法区分。这些结果强化了早期的发现，即钙诱导的肌原纤维收缩的丧失不是周期性麻痹瘫痪的原因。
Lateral gastrocnemius muscle biopsies from a 26‐year‐old man with hyperkalemic periodic paralysis and a 23‐year‐old man with hypokalemic periodic paralysis were studied. Both patients came from families in which older relatives had developed a vacuolar myopathy in association with their periodic paralysis. Muscle fibers were chemically skinned, and individual fibers were studied with a low‐compliance strain gauge. The tension generated by fibers was studied in baths with calcium concentrations from 10‐8mol/L to 2.5 × 10‐5mol/L. The Ca‐tension relationships and maximal tensions (normalized to fiber cross‐sectional area) of fast and slow twitch fibers were indistinguishable from those found in fibers from 5 normal subjects. The results reinforce earlier findings which suggested that loss of Ca‐induced myofibril contraction was not the cause of paralysis in periodic paralysis.