In Vitro Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion.
In Vitro Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion.
复制标题
使用铂纳米粒子和 Nafion 修饰的掺硼金刚石微电极体外监测小鼠结肠中一氧化氮的释放。
DOI：
10.1021/acs.analchem.2c03731
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发表时间：
2023
影响因子：
7.4
通讯作者：
Swain,GregM
中科院分区：
文献类型：
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作者：
Henderson,Skye;Bhardwaj,Kirti;Perugachi,Victoria;Espinoza-Montero,Patricio;Galligan,JamesJ;Swain,GregM
本研究报道了一种铂纳米颗粒和Nafion修饰的硼掺杂金刚石微电极的制备及其在体外小鼠结肠中检测一氧化氮（NO）的应用。铂纳米颗粒沉积是使用2.0 mmol L-1六氯铂酸钾溶液进行的，并在0.01 V s-1下从-0.2至1.3 VvsAg/AgCl循环10次。通过浸入含有2.5%（w/v）胶体Nafion的溶液中并在潮湿环境中在55 °C下干燥过夜来施加Nafion覆盖层。根据电极对NO氧化的响应以及对生物介质中NO电化学检测的主要干扰物亚硝酸根（NO2-）氧化的抑制作用，选择了最佳的微电极制备条件。检测灵敏度为16.7 ± 2.7 mA M ~（-1）cm ~（-2）（n= 3电极），检出限为0.5 μmol L ~（-1）（S/N = 3），电极响应重现性为2.5%（RSD）。电刺激和连续安培法被用来测量NO释放从肌间神经节在野生型雄性和雌性小鼠在响应越来越多的电刺激，研究在结肠中的氮能信号。我们还提供了关于使用光遗传学选择性刺激氮能肌间神经元使用蓝光刺激的初步数据，目的是了解抑制性神经肌肉信号传导如何参与控制肠道运动的肌间神经丛回路。
This research reports on the preparation of a boron-doped diamond microelectrode modified with platinum nanoparticles and Nafion and its application for detecting nitric oxide (NO)in vitroin the mouse colon. Platinum nanoparticle deposition was performed potentiodynamically using a 2.0 mmol L–1potassium hexachloroplatinate solution and cycling from −0.2 to 1.3 VvsAg/AgCl at 0.01 V s–1for 10 cycles. The Nafion overlayer was applied by immersion in a solution containing 2.5% (w/v) colloidal Nafion and drying overnight at 55 °C in a humid environment. The optimal microelectrode preparation conditions were chosen based on the electrode response for NO oxidation as well as rejection of nitrite (NO2–) oxidation, the main interferent in the electrochemical detection of NO in biological media. Detection figures of merit include a sensitivity of 16.7 ± 2.7 mA M–1cm–2(n= 3 electrodes), a detection limit of 0.5 μmol L–1(S/N = 3), and an electrode response reproducibility of 2.5% (RSD). Electrical stimulation and continuous amperometry were used to measure NO release from myenteric ganglia in wild-type male and female mice in response to an increasing number of electrical stimuli to study nitrergic signaling in the colon. We also present preliminary data regarding the use of optogenetics to selectively stimulate nitrergic myenteric neurons using blue light stimulation with a goal of understanding how inhibitory neuromuscular signaling is involved in the myenteric plexus circuitry that controls intestinal motility.