Superantigen Genes Are More Important than the emm Type for the Invasiveness of Group A Streptococcus Infection
Superantigen Genes Are More Important than the emm Type for the Invasiveness of Group A Streptococcus Infection
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DOI：
10.1086/653082
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发表时间：
2010-07-01
影响因子：
6.4
通讯作者：
Rink, Lothar
中科院分区：
文献类型：
--
作者：
Lintges, Maria;van der Linden, Mark;Rink, Lothar
背景在这项研究中，我们研究了超抗原基因和emm基因型的作用，临床化脓性链球菌分离株收集在德国之间的1997年和2003年。采用多重聚合酶链反应检测所有11个已知的超抗原基因，并对emm基因进行测序。使用两步探索性数据分析程序，我们发现，在超抗原基因和emm类型的联合分析后，只有超抗原基因E11-E13，spem和E14对侵袭性有预测价值，比值比分别为7.992，3.209和2.323。由于emm类型与高度预测性超抗原基因之间存在关联，因此联合分析emm 1的侵袭性预测值丢失。另一方面，超抗原基因ssa和emm 77的存在是非侵袭性的预测因子，比值比分别为0.370和0.271。这些结果表明，超抗原基因的存在对A组链球菌感染的侵袭性比emm型更重要，可能是宿主高危HLA型与病原体之间的联系。此外，我们发现了一个非常明确的相关性之间的存在的基因emm 1的存在，emm 1和侵袭性之间的关系是基于超抗原基因谱的基因。我们的数据表明，超抗原基因谱是非常重要的A组链球菌感染的临床结果。
Background. In this study, we examined the role of superantigen genes and emm genotypes of clinical Streptococcus pyogenes isolates collected in Germany between 1997 and 2003.Methods. Multiplex polymerase chain reaction for all 11 currently known superantigen genes and sequencing for emm types were used.Results. Using a 2-step explorative data analysis procedure, we found that after combined analysis of superantigen genes and emm types, only the superantigen genes spea1-spea3, spem, and spea4 have a predictive value for invasiveness, with odds ratios of 7.992, 3.209, and 2.323, respectively. The predictive value for invasiveness of emm1 was lost after combined analysis because of the association between emm type and the highly predictive superantigen genes. On the other hand, presence of the superantigen gene ssa and of emm77 are predictors of noninvasiveness, with odds ratios of 0.370 and 0.271, respectively.Conclusions. These results indicate that the presence of superantigen genes is more important for the invasiveness of group A Streptococcus infection than emm type and may be the connection between the high-risk HLA type of the host and the pathogen. Furthermore, we found a very clear correlation between the presence of the genes spea1-spea3 and the presence of the gene emm1, which indicates that the relationship between emm1 and invasiveness is based on the superantigen gene profile. Our data suggest that the superantigen gene profile is of high importance for the clinical outcome of group A Streptococcus infections.