The complete genome sequence of Bacillus velezensis LPL061, an exopolysaccharide-producing bacterium
The complete genome sequence of Bacillus velezensis LPL061, an exopolysaccharide-producing bacterium
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产胞外多糖细菌贝莱斯芽孢杆菌 LPL061 的完整基因组序列
DOI：
10.1007/s13205-020-02228-y
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发表时间：
2020-05
期刊：
影响因子：
2.8
通讯作者：
Li Pinglan
中科院分区：
文献类型：
--
作者：
Wu Ruiyun;Qin Yuxuan;Shen Qian;Li Pinglan
Bacillus velezensisLPL061, which shows strong exopolysaccharide (EPS) producing capacity, was isolated from carnations in Beijing, China. The complete genome of LPL061 comprised a single circular chromosome (3,907,268 bp; G+C content of 46.7%) with 3,737 coding DNA sequences, 26 rRNA, and 89 tRNA. According to genome analysis, 12 protein-coding genes which related to polysaccharide biosynthesis in LPL061 were identified. Comparative genome analysis revealed that the EPS biosynthetic gene cluster was relatively conserved amongBacillusspecies. EPS showed approximately 60% inhibitory activity on the α-glucosidase at 100 μg/mL. The results of quantitative reverse transcription PCR further demonstrated that compared to insulin-resistant model with insulin (500 μg/mL) (without EPS treatment), the insulin-resistant HepG2 cells treated with EPS decreased the expression of phosphoenolpyruvate carboxykinase (PEPCK) from 4.425 to 0.1587, glucose-6-phosphatase (G6Pase) decreased from 4.272 to 0.1929, and glycogen synthase kinase3β (GSK(3)β) decreased from 2.451 to 0.993, respectively. Meanwhile, EPS treatment increased GS expression and resulted in intracellular glycogen concentration increased from 28.30% to 86.48%, which further supported that EPS form LPL061 could reduce the concentration of blood glucose effectively. These results could be beneficial for better understanding of the hypoglycemic mechanism ofB. velezensisLPL061 EPS and developing an EPS-based anti-diabetic agent in the future.
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影响因子：
--
作者：
Rangika BS;Dayananda PD;Peiris DC
通讯作者：
Peiris DC
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
6.1
作者：
Hui Teng;Lei Chen;Hongbo Song
通讯作者：
Hui Teng;Lei Chen;Hongbo Song
影响因子：
5.9
作者：
Yu, Rui;Zuo, Fanglei;Chen, Shangwu
通讯作者：
Chen, Shangwu
影响因子：
5
作者：
Yang, Zongchun;Huang, Wei;Wang, Xiaowan
通讯作者：
Wang, Xiaowan