Impact of Engineered Si Nanoparticles on Seed Germination, Vigour Index and Genotoxicity Assessment via DNA Damage of Root Tip Cells in Lens culinaris
Impact of Engineered Si Nanoparticles on Seed Germination, Vigour Index and Genotoxicity Assessment via DNA Damage of Root Tip Cells in Lens culinaris
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工程硅纳米颗粒对种子萌发、活力指数的影响以及通过对小菜籽根尖细胞 DNA 损伤进行遗传毒性评估
DOI：
10.4172/2329-9029.1000218
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Z. Khan
中科院分区：
文献类型：
--
作者：
Z. Khan
工程纳米颗粒因其在生物医学、农业、光学和电子领域的广泛潜在应用而具有巨大的科学意义。本研究旨在研究硅纳米颗粒（SiNP）对植物生长参数的影响，并检测它们可能引起的遗传毒性。种子萌发结果表明，较低浓度的SiNP可促进种子萌发、活力指数和生物量；然而，在较高浓度下，它们显示出偏差的结果。为了研究毒理学终点，对根尖细胞进行了显微镜检查。结果表明，接触纳米颗粒会显着增加染色体畸变的数量。与对照相比，在治疗组中观察到有丝分裂指数（MI）的剂量依赖性下降。结果表明，纳米颗粒有可能通过损害有丝分裂和诱导染色体异常来改变 DNA，从而导致基因组不稳定。较低浓度的硅纳米颗粒可以对扁豆的发芽和生物量产生积极的结果。硅纳米颗粒可以通过种子或叶面施肥渗透到组织系统中，干扰细胞分裂周期，诱导纳米毒性。
Engineered nanoparticles are of great scientific interest due to their wide variety of potential applications in biomedical, agricultural, optical and electronic fields. The present study has been designed to study the effect of Si nanoparticle (SiNP) on plant growth parameters and to detect the possible genotoxicity induced by them. Seed germination results indicated that SiNP at lower concentration promotes seed germination, Vigour index and biomass; however, at higher concentrations they showed deviated results. To study toxicological end points microscopic examination of root tip cells were carried out. The result showed that exposure to the nanoparticle increase the number of chromosomal aberrations significantly. Dose-dependent decrease in Mitotic index (MI) in the treated populations was observed as compared to control. The result suggests potential of nanoparticle in causing genomic instability by impairing mitosis and altering DNA by inducing chromosomal anomalies. Lower concentration of Si NP can induce positive results on germination and biomass of lentils Si Nanoparticles can penetrate the tissue system through seed or foliar dressing and interfere with the cell division cycle, inducing nanotoxicity.