Properties and modification of porous 3-D collagen/hydroxyapatite composites
Properties and modification of porous 3-D collagen/hydroxyapatite composites
复制标题
DOI：
10.1016/j.ijbiomac.2012.10.002
复制
发表时间：
2013-01-01
影响因子：
8.2
通讯作者：
Kozlowska, J.
中科院分区：
文献类型：
--
作者：
Sionkowska, A.;Kozlowska, J.
采用冷冻干燥技术制备了多孔三维胶原基质，并通过添加不同量的纳米羟基磷灰石对其进行了改性。对于化学交联，使用EDC/NHS。物理交联通过脱水热处理实现。研究了交联后材料的力学性能、形貌、溶解性、孔隙率、密度、酶降解性和溶胀性能。随着复合支架中羟基磷灰石和胶原含量的增加，支架的密度和压缩模量增加，而溶胀率和孔隙率降低。所研究的支架在PBS溶液中缓慢溶解。在暴露于胶原酶后，DHT交联胶原基质显示出比EDC交联样品快得多的降解速率。(c)2012爱思唯尔有限公司版权所有。
A freeze drying technique was used to form porous three-dimensional collagen matrixes modified by the addition of a variable amount of nano-hydroxyapatite. For chemical cross-linking EDC/NHS were used. Physical cross-linking was achieved by dehydrothermal treatment. Mechanical properties, morphology, dissolution, porosity, density, enzymatic degradation and swelling properties of materials have been studied after cross-linking. The density of scaffolds and its compressive modulus increased with an increasing amount of hydroxyapatite and collagen concentration in the composite scaffold, while the swelling ratio and porosity decreased. The studied scaffolds dissolved slowly in PBS solution. DHT cross-linked collagen matrices showed a much faster degradation rate after exposure to collagenase than the EDC cross-linked samples. (c) 2012 Elsevier B.V. All rights reserved.