Comparison of human stem cells derived from various mesenchymal tissues - Superiority of synovium as a cell source
Comparison of human stem cells derived from various mesenchymal tissues - Superiority of synovium as a cell source
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DOI：
10.1002/art.21212
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发表时间：
2005-08-01
影响因子：
--
通讯作者：
Muneta, T
中科院分区：
文献类型：
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作者：
Sakaguchi, Y;Sekiya, I;Muneta, T
Objective.比较从骨髓、滑膜、骨膜、骨骼肌和脂肪组织中分离的人间充质干细胞（MSCs）的特性。在膝关节韧带损伤手术期间从8个供体获得人间充质组织。在胶原酶消化或梯度密度分离后，将有核细胞以适当的密度铺板，以最大速率扩增，而没有集落与集落接触。比较5种不同组织来源的MSCs的产量、扩增能力、分化潜能和表位特征。与其他间充质组织相比，骨髓中每10（3）个有核细胞的集落数较低，而每个集落的细胞数较高。当细胞以低密度每14天重新铺板时，骨髓、滑膜和骨膜来源的细胞甚至在第10代时仍保持其增殖能力。在软骨形成的研究中，细胞在体外形成颗粒并培养，来自骨髓、滑膜和骨膜来源的细胞的颗粒被证明更大，并且对软骨基质的染色更广泛。特别是滑膜衍生的细胞，具有最大的软骨形成能力。在脂肪形成实验中，油红O-阳性集落的频率在滑膜和脂肪组织来源的细胞中最高。在骨生成的研究中，茜素红阳性集落的比率在骨髓、滑膜和骨膜来源的细胞中最高。对于表位分析，测量了15种表面抗原。无论细胞来源如何，大多数似乎具有相似的表位谱。我们的研究结果表明，有显着的差异，MSC的属性根据组织来源，超越供体和实验变异。证实了滑膜作为临床应用的MSC的潜在来源的优越性。
Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Methods. Human mesenchymal tissues were obtained from 8 donors during knee surgery for ligament injury. After collagenase digestion or gradient-density separation, nucleated cells were plated at an appropriate density for expansion at the maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from the 5 different tissue sources.Results. Colony number per 10(3) nucleated cells was lower, and cell number per colony was higher, in bone marrow than in other mesenchymal tissues. When the cells were replated at low density every 14 days, bone marrow-, synovium-, and periosteum-derived cells retained their proliferation ability even at passage 10. In chondrogenesis studies in which the cells were pelleted and cultured in vitro, pellets from bone marrow-, synovium-, and periosteum-derived cells were shown to be larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. In adipogenesis experiments, the frequency of oil red O-positive colonies was highest in synovium- and adipose tissue-derived cells. In studies of osteogenesis, the rate of alizarin red-positive colonies was highest in bone marrow-, synovium-, and periosteum-derived cells. For epitope profiling, 15 surface antigens were measured. Most appeared to have similar epitope profiles irrespective of cell source.Conclusion. Our findings indicate that there are significant differences in MSC properties according to tissue source, beyond donor and experimental variation. Superiority of synovium as a potential source of MSCs for clinical applications was demonstrated.