FLICKING THE SWITCHES - PHOSPHORYLATION OF SERINE/THREONINE PROTEIN PHOSPHATASES
FLICKING THE SWITCHES - PHOSPHORYLATION OF SERINE/THREONINE PROTEIN PHOSPHATASES
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DOI：
10.1006/scbi.1995.0028
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发表时间：
1995-08-01
影响因子：
14.5
通讯作者：
BRAUTIGAN, DL
中科院分区：
文献类型：
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作者：
BRAUTIGAN, DL
信号转导涉及蛋白质磷酸化和去磷酸化。要产生实质性和短暂的变化，需要激酶和磷酸酶的协调和相互调节。实现这一点的一种机制是磷酸化，并且现在有关于所有主要类型的蛋白质Ser/Thr磷酸酶的磷酸化的报道。1型和2A型磷酸酶的磷酸化发生在特征性C-末端序列内，并导致磷酸酶活性丧失。磷酸酶催化分子内去磷酸化，并恢复活性。这种性质可能解释了细胞和组织提取物中磷酸酶的表观组成活性。磷酸酶的磷酸化是在生长周期期间和响应刺激而在细胞中关闭和打开活性的一种方式。
Signal transduction involves protein phosphorylation and dephosphorylation. To produce both substantial and transient changes requires coordinated and reciprocal regulation of kinases and phosphatases. One mechanism to accomplish this is phosphorylation, and there are now reports of phosphorylation of all the major types of protein Ser/Thr phosphatases. Phosphorylation of type-1 and type-2A phosphatases occurs within characteristic C-terminal sequences and results in the loss of phosphatase activity. The phosphatases catalyse intramolecular dephosphorylation, with a restoration of activity. This property probably accounts for the apparent constitutive activity of phosphatases in cell and tissue extracts. Phosphorylation of phosphatases is a way to flick the activity off and on in cells during the growth cycle and in response to stimuli.