PulseNet USA:: A five-year update
PulseNet USA:: A five-year update
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DOI：
10.1089/fpd.2006.3.9
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Swaminathan, B.
中科院分区：
文献类型：
--
作者：
Gerner-Smidt, P.;Hise, K.;Swaminathan, B.
PulseNet USA 是美国食源性感染的分子监测网络。自 1996 年成立以来，它在检测、调查和控制由产志贺毒素大肠杆菌 O157:[H7] (STEC O157)、肠沙门氏菌、单核细胞增生李斯特氏菌、志贺氏菌属和弯曲杆菌引起的众多疫情方面发挥了重要作用。本文描述了网络的当前状态，包括所使用的方法及其未来的可能性。目前网络中首选的亚型分型方法是脉冲场凝胶电泳 (PFGE)，这是一种经过验证的高度辨别性的分子亚型分型方法。新的更简单的基于序列的亚型分型方法正在开发和验证中，以补充并最终取代 PFGE。 PulseNet本质上是一个集群检测网络，但系统中的数据现在也将用于零星感染的归因分析。 PulseNet 平台还将用作准备和应对食品生物恐怖主义行为的主要工具。
PulseNet USA is the molecular surveillance network for foodborne infections in the United States. Since its inception in 1996, it has been instrumental in detection, investigation and control of numerous outbreaks caused by Shiga toxin-producing Escherichia coli O157:[H7] (STEC O157), Salmonella enterica, Listeria monocytogenes, Shigella spp., and Campylobacter. This paper describes the current status of the network, including the methodologies used and its future possibilities. The currently preferred subtyping method in the network is pulsed-field gel electrophoresis (PFGE), a proven highly discriminatory molecular subtyping method. New simpler sequence-based subtyping methods are under development and validation to complement and eventually replace PFGE. PulseNet is essentially a cluster detection network, but the data in the system will now also be used in attribution analyses of sporadic infections. The PulseNet platform will also be used as a primary tool in preparedness and response to acts of food bioterrorism.