Suppression of adipocyte differentiation by Cordyceps militaris through activation of the aryl hydrocarbon receptor.
Suppression of adipocyte differentiation by Cordyceps militaris through activation of the aryl hydrocarbon receptor.
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蛹虫草通过激活芳基烃受体抑制脂肪细胞分化。
DOI：
10.1152/ajpendo.90373.2008
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
and Kitamura M
中科院分区：
文献类型：
--
作者：
Shimada T;Hiramatsu N;Kasai A;Mukai M;Okamura M;Yao J;Huang T;Tamai M;Takahashi S;Nakamura T;and Kitamura M
菌丝体提取物具有广泛的生物活性，可调节哺乳动物细胞的功能。在这份报告中，我们试图确定与使用3 T3-L1细胞的抗脂肪形成菌丝体，并发现蛹虫草提取物专门抑制3 T3-L1前脂肪细胞分化为成熟脂肪细胞，而不影响细胞活力。这种抑制作用具有剂量依赖性、可逆性，并与1）脂质蓄积减少，2）脂肪细胞标志物（包括脂联素、过氧化物酶体增殖物激活受体-γ和CCAAT/增强子结合蛋白-α）的钝性诱导，以及3）前脂肪细胞标志物单核细胞趋化蛋白-1（monocyte chemoattractant protein-1）的持续表达相关。militarin还显著降低成熟脂肪细胞中的脂质积累和肥大，并在长期培养期间保持它们对胰岛素的反应（Akt的磷酸化）。随后的实验表明，C.因此，军事化具有激活芳烃受体（AhR）的潜力。在3 T3-L1细胞中，用AhR激动剂包括苯并[a]芘和3-甲基胆蒽处理再现了C.军队。此外，AhR的显性负性抑制可消除C.对脂肪细胞分化的影响。这些结果表明C. militarishas通过激活AhR干扰脂肪细胞分化的潜力。
Mycelial extracts have a wide range of biological activities that modulate functions of mammalian cells. In this report, we sought to identify antiadipogenic mycelia with the use of 3T3-L1 cells and found that the extract ofCordyceps militarisexclusively suppressed differentiation of 3T3-L1 preadipocytes into mature adipocytes without affecting cell viability. This inhibitory effect was dose dependent, reversible, and associated with1) a decrease in lipid accumulation,2) blunted induction of adipocyte markers including adiponectin, peroxisome proliferator-activated receptor-γ, and CCAAT/enhancer binding protein-α, and3) sustained expression of a preadipocyte marker, monocyte chemoattractant protein-1.C. militarisalso significantly decreased accumulation of lipid and hypertrophy in mature adipocytes and preserved their response to insulin (phosphorylation of Akt) during prolonged culture. Subsequent experiments revealed thatC. militarishas the potential to activate the aryl hydrocarbon receptor (AhR). In 3T3-L1 cells, treatment with AhR agonists including benzo[a]pyrene and 3-methylcholanthrene reproduced the antiadipogenic effect ofC. militaris. Furthermore, dominant-negative inhibition of AhR abrogated the suppressive effect ofC. militarison adipocyte differentiation. These results suggest thatC. militarishas the potential to interfere with adipocyte differentiation through activation of AhR.