Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells.
Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells.
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胰岛细胞表达破伤风毒素和单克隆抗体 A2B5 受体。
DOI：
10.1073/pnas.79.16.5066
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发表时间：
1982
影响因子：
11.1
通讯作者：
Wells,S
中科院分区：
文献类型：
--
作者：
Eisenbarth,GS;Shimizu,K;Bowring,MA;Wells,S
对抗体A2B5和破伤风毒素与胰岛细胞、胰岛细胞瘤和其他人类胺前体摄取和脱羧化(APUD)肿瘤的反应进行了描述。间接免疫荧光显示抗体A2B5和破伤风毒素可与人、大鼠、鸡和小鼠胰岛细胞的质膜特异性结合。抗体A2B5与活的胰岛细胞表面的结合使这些细胞能够通过使用荧光激活的细胞分选器从胰腺细胞悬液中分离出来。在旨在确定破伤风毒素和抗体A2B5是否与同一表面抗原结合的研究中，A2B5和破伤风毒素不竞争结合正常胰岛细胞、人类胰岛细胞瘤或大鼠胰岛细胞瘤。除了与胰岛细胞肿瘤结合外，抗体A2B5还与冰冻切片、分离的细胞以及神经、神经脊和APUD来源的细胞系发生反应。
Studies of the reaction of antibody A2B5 and tetanus toxin with pancreatic islet cells, islet cell tumors, and other human amine precursor uptake and decarboxylation (APUD) tumors are described. By indirect immunofluorescence, antibody A2B5 and tetanus toxin were shown to specifically bind to the plasma membrane of human, rat, chicken, and mouse islet cells. The binding of antibody A2B5 to the cell surface of living islet cells has allowed isolation of these cells from a suspension of pancreatic cells by using a fluorescence-activated cell sorter. In studies designed to determine whether tetanus toxin and antibody A2B5 bound to the same surface antigen, A2B5 and tetanus toxin did not compete for binding to normal islet cells, a human islet cell tumor, or a rat islet cell tumor. In addition to binding to islet cell tumors, antibody A2B5 reacts with frozen sections, isolated cells, and cell lines of neural, neural crest, and APUD origin.