Optically modulated fluorophores for selective fluorescence signal recovery.

Optically modulated fluorophores for selective fluorescence signal recovery.
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DOI:
10.1021/ja809785s
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发表时间:
2009-04-08
影响因子:
15
通讯作者:
Dickson RM
Dickson RM
中科院分区:
化学1区
文献类型:
--
作者:
Richards CI;Hsiang JC;Senapati D;Patel S;Yu J;Vosch T;Dickson RM

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生物科学中的荧光成像受到显著的深度依赖性信号衰减和高荧光背景的阻碍。我们已经开发了光学调制的近红外发射的少原子银纳米点,选择性地和动态地photobliquely同时激发与二次激光,使高灵敏度的图像提取,揭示只有解调的荧光团。在高背景环境中,图像解调被证明可以从完全模糊的背景发射中提取微弱信号。
Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and high fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser enabling high sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high background environments to extract weak signals from completely obscuring background emission.
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