Regulation of phagocytosis against bacterium by Rab GTPase in shrimp Marsupenaeus japonicus
Regulation of phagocytosis against bacterium by Rab GTPase in shrimp Marsupenaeus japonicus
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DOI：
10.1016/j.fsi.2008.05.006
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发表时间：
2008-09-01
影响因子：
4.7
通讯作者：
Zhang, Xiaobo
中科院分区：
文献类型：
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作者：
Zong, Rongrong;Wu, Wenlin;Zhang, Xiaobo
Rab GTP酶是Ras超家族的成员，在吞噬体的形成和成熟过程中发挥重要作用。然而，Rab蛋白参与甲壳动物对入侵病原体的吞噬作用仍然未知。本研究利用RNAi和mRNA过表达技术研究了对虾Rab基因（PjRab）在血细胞吞噬细菌过程中的功能。结果表明，当PjRab基因被序列特异性siRNA沉默后，对FITC标记的副溶血弧菌的吞噬百分率和吞噬指数显著降低，表明PjRab蛋白在血细胞吞噬中是必需的。另一方面，PjRab基因的过表达导致吞噬百分率和吞噬指数的增加。结果表明，PjRab蛋白参与了对虾血细胞吞噬功能的调节。我们的报告的调节吞噬作用的Rab GTdR将有助于更好的线索，以实现仍然知之甚少的分子事件涉及虾以及甲壳类动物的免疫反应。(c)2008爱思唯尔有限公司保留所有权利。
Rab GTPases, members of the Ras superfamily, play important roles in phagosome formation and maturation. However, the involvement of Rab protein in phagocytosis against invading pathogens in crustacean remains unknown. In the present study, the RNAi and mRNA overexpression assays were conducted to elucidate the function of shrimp Rab gene (designated as PjRab) in hemocytic phagocytosis against bacterium. The results indicated that the phagocytic percentage and phagocytic index using FITC-labeled Vibrio parahemolyticus were significantly decreased when the PjRab gene was silenced by sequence-specific siRNA, suggesting that the PjRab protein was essential in hemocytic phagocytosis. On the other hand, the overexpression of PjRab gene leaded to the increase of phagocytic percentage and phagocytic index. The findings indicated that the PjRab protein was involved in the regulation of hemocytic phagocytosis of shrimp. Our report on the regulation of phagocytosis by Rab GTPase would contribute a better clue to realize the still poorly understood molecular events involved in shrimp as well as crustacean immune response. (c) 2008 Elsevier Ltd. All rights reserved.