Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro.
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro.
复制标题
添加不含雄激素的附睾蛋白提取物可增加未成熟仓鼠精子体内和体外受精的能力。
DOI：
10.1530/jrf.0.0710433
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
J. Blaquier
中科院分区：
文献类型：
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作者：
Fernanda González Echeverría;P. Cuasnicú;A. Piazza;L. Piñeiro;J. Blaquier
采用活体授精法，对仓鼠附睾不同节段精子的受精能力进行了测定。头和近侧体精子不能生育;来自远侧附睾体的精子受精率为13%（38/290），来自近侧和远侧附睾尾的精子分别为71%和87%。当体外受精测试时，远端体精子穿透44%的卵母细胞，而远端尾精子受精87%的卵母细胞。在培养基BMOC中回收的远端体精子在体内受精率为13%（28/219），而与附睾蛋白制剂（0.8 mg蛋白/ml）混合的精子受精率为24%（49/204; P <0.01）。用0.8mg附睾蛋白制剂与精子体远端进行体内授精，受精率为34%（31/90），而用去势30 d的附睾蛋白制剂进行体内授精，受精率仅为22%（23/103; P <0.05）。当远端精子体在无（对照）或含蛋白质制剂（0.8或1.6 mg蛋白质/ml）的培养基中预孵育5 h时，发现蛋白质浓度为1.6 mg/ml时，体外卵母细胞穿透率显著增加（25与45%相比; P <0.05）。这些结果证实并扩展了先前的观察结果，表明附睾分泌的雄激素依赖性糖蛋白在精子成熟过程中获得可复性中的作用。
The fertility of spermatozoa from the different epididymal segments of hamsters was tested by in-vivo insemination. Caput and proximal corpus spermatozoa were non-fertile; spermatozoa from the distal corpus epididymidis fertilized 13% (38/290) oocytes and those from the proximal and distal cauda epididymidis 71 and 87%, respectively. When tested by in-vitro insemination, distal corpus spermatozoa penetrated 44% of oocytes while those from the distal cauda fertilized 87% of oocytes. Spermatozoa from the distal corpus recovered in Medium BMOC fertilized 13% (28/219) of oocytes in vivo, while those mixed with an epididymal protein preparation (0.8 mg protein/ml) fertilized 24% (49/204; P less than 0.01) of oocytes. When distal corpus spermatozoa were inseminated in vivo with 0.8 mg epididymal protein preparation 34% (31/90) oocytes were fertilized and only 22% (23/103; P less than 0.05) oocytes were fertilized when the proteins were obtained from epididymides of animals castrated for 30 days. When distal corpus spermatozoa were preincubated for 5 h in medium without (control) or with protein preparation (0.8 or 1.6 mg protein/ml), a significant increase in in-vitro oocyte penetration was found (25 compared with 45%; P less than 0.05) when the protein was present at 1.6 mg/ml. These results confirm and extend previous observations suggesting a role for androgen-dependent glycoproteins secreted by the epididymis in the acquisition of fertilizing ability that occurs during sperm maturation.