Clinical significance of MicoRNA-155 expression in human breast cancer
Clinical significance of MicoRNA-155 expression in human breast cancer
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DOI：
10.1002/jso.22153
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Tang, Jin-Hai
中科院分区：
文献类型：
--
作者：
Chen, Jian;Wang, Bing-Chan;Tang, Jin-Hai
本研究旨在探讨miR-155在乳腺癌中的表达及其临床意义。方法采用TaqMan实时荧光定量RT-PCR方法检测乳腺癌组织中miR-155的表达。分析miR-155表达与乳腺癌患者临床病理因素及预后的相关性。然后，通过单因素和多因素分析来分析miR-155表达的预后价值。并通过反义技术检测miR-155表达对乳腺癌细胞表型的影响。结果乳腺癌组织中miR-155的相对表达量显著高于相应的非肿瘤组织。miR-155高表达与肿瘤分级、分期及淋巴结转移有关（P = 0.001）。0.012、0.001和0.003）。KaplanMeier生存分析显示高miR-155组的无病生存率和总生存率均显著低于低miR-155组（P = 0.001）。0.038和0.029）。多因素分析显示miR-155高表达是预后不良的因素（P = 0.001）。0.009）。此外，反义miR-155可抑制乳腺癌细胞的生长，诱导细胞阻滞于G 0/G1期，促进细胞凋亡，并提高乳腺癌细胞的放射敏感性。结论miR-155表达可能是乳腺癌的一个独立的预后因子，并可作为乳腺癌治疗的靶点。外科肿瘤学杂志2012; 106：260266. (c)2011 Wiley Periodicals，Inc.
Background The aim of this study is to investigate clinical significance of miR-155 expression in breast cancer. Methods TaqMan real-time RT-PCR was performed to detect miR-155 expression in breast cancer tissues. The correlation of miR-155 expression with clinicopathological factors and prognosis of breast cancer patients was analyzed. Then, the prognostic value of miR-155 expression was analyzed by univariate and multivariate analyses. Moreover, the effect of miR-155 expression on phenotypes of breast cancer cell was determined by antisense technology. Results The relative expression of miR-155 was significantly higher in breast cancer tissues than in corresponding nontumor tissues. High miR-155 expression was correlated with higher tumor grade, advanced tumor stage and lymph node metastasis (P?=?0.012, 0.001, and 0.003, respectively). KaplanMeier survival analysis indicated that the disease-free and overall survival rates of high miR-155 group were significantly lower than those of low miR-155 group (P?=?0.038 and 0.029, respectively). Multivariate analysis showed that high miR-155 expression was a poor prognostic factor (P?=?0.009). Furthermore, antisense targeting miR-155 could inhibit growth, induce cell arrest in G0/G1 phase, enhance apoptosis, and increase radiosensitivity in breast cancer cells. Conclusions MiR-155 expression might be an independent prognostic factor and a therapeutic target for human breast cancer. J. Surg. Oncol. 2012; 106:260266. (c) 2011 Wiley Periodicals, Inc.