Articular cartilage chondrocytes in type I and type II collagen-GAG matrices exhibit contractile behavior in vitro
Articular cartilage chondrocytes in type I and type II collagen-GAG matrices exhibit contractile behavior in vitro
复制标题
DOI：
10.1089/107632700750022198
复制
发表时间：
2000-10-01
影响因子：
--
通讯作者：
Spector, M
中科院分区：
文献类型：
--
作者：
Lee, CR;Breinan, HA;Spector, M
关节软骨缺陷通常不会自然愈合，未经治疗的病变可能会使关节易患骨关节炎。为了促进软骨缺损的愈合，许多研究人员正在转向涉及培养细胞和/或多孔、可吸收基质的组织工程方法。本研究研究了接种在多孔胶原蛋白-糖胺聚糖 (GAG) 支架中的培养犬软骨细胞的收缩行为。从成年犬的膝关节中分离并在单层培养中扩增的软骨细胞被接种到多孔胶原-GAG支架中。支架具有两种不同的成分，主要胶原蛋白是 I 型或 TI 型胶原蛋白，并且具有不同的孔径。在 4 周的培养期内，尽管硬度范围很广（I 型支架为 145 +/- 23 Pa，II 型材料为 732 +/- 35 Pa），但接种的细胞收缩了所有基于 I 型和 II 型胶原的基质。孔径（25-85μm，I型；53-257μm，II型）不影响细胞介导的收缩。免疫组织化学染色显示，在种子细胞的细胞质和正常成年犬关节软骨的软骨细胞中存在α-平滑肌肌动蛋白，这是一种负责平滑肌细胞和肌成纤维细胞收缩的亚型。
Natural healing of articular cartilage defects generally does not occur, and untreated lesions may predispose the joint to osteoarthritis. To promote healing of cartilage defects, many researchers are turning toward a tissue engineering approach involving cultured cells and/or porous, resorbable matrices. This study investigated the contractile behavior of cultured canine chondrocytes seeded in a porous collagen-glycosaminoglycan (GAG) scaffold. Chondrocytes isolated from the knee joints of adult canines and expanded in monolayer culture were seeded into porous collagen-GAG scaffolds. Scaffolds were of two different compositions, with the predominant collagen being either type I or type TI collagen, and of varying pore diameters, Over the 4-week culture period, the seeded cells contracted all of the type I and type II collagen-based matrices, despite a wide range of stiffness (145 +/- 23 Pa, for the type I scaffold, to 732 +/- 35 Pa, for the type II material). Pore diameter (25-85 mum, type I; and 53-257 mum, type II) did not affect cell-mediated contraction. Immunohistochemical staining revealed the presence of alpha -smooth muscle actin, an isoform responsible for contraction of smooth muscle cells and myofibroblasts, in the cytoplasm of the seeded cells and in chondrocytes in normal adult canine articular cartilage.