Reproducibility of single-subject functional connectivity measurements.
Reproducibility of single-subject functional connectivity measurements.
复制标题
DOI：
10.3174/ajnr.a2330
复制
发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Yurgelun-Todd D
中科院分区：
文献类型：
--
作者：
Anderson JS;Ferguson MA;Lopez-Larson M;Yurgelun-Todd D
静息状态功能连接的测量已越来越多地用于表征神经病理和神经发育人群。我们收集的数据，以表征多少成像时间是必要的，以获得一个可靠的单一主题的诊断测试所需的可重复的定量功能连接测量。我们对一个受试者进行了100次5分钟的BOLD扫描，分为10次扫描，每次扫描10次，受试者在休息或观看卡通视频剪辑时。这些数据进行了比较，从36个正常对照受试者的静息状态BOLD扫描，通过评估每对64个小的球形ROI之间的相关性，从发表的功能parcellation的大脑。单个受试者和组数据收敛到与1/sqrt（n）成比例的个体和群体连接值的可靠估计值。使用长达25分钟的成像时间，可靠性得到显著改善，额外时间的改善较小。所研究的个体和群体的功能连接“指纹”在约15分钟的成像时间开始发散，即使在4小时的成像时间也具有增加的可靠性。在我们的受试者休息或观看动画片期间，分类器区分扫描在10分钟时准确率为95%，在15分钟成像时间时准确率为100%。个体受试者和对照人群收敛到可靠的、不同的功能连接性概况，这些功能连接性概况是任务调制的，并且可以用足够的成像时间来区分。我们建议，如果可行，定量单受试者诊断测试或分类使用至少25分钟的BOLD成像时间。
Measurements of resting state functional connectivity have increasingly been used for characterization of neuropathological and neurodevelopmental populations. We collected data to characterize how much imaging time is necessary to obtain reproducible quantitative functional connectivity measurements needed for a reliable single-subject diagnostic test. We obtained 100 five-minute BOLD scans on a single subject, divided into 10 sessions of 10 scans each, with the subject at rest or while watching video clips of cartoons. These data were compared to resting state BOLD scans from 36 normal control subjects by evaluating correlation between each pair of 64 small spherical ROI's obtained from a published functional parcellation of the brain. Single-subject and group data converged to reliable estimates of individual and population connectivity values proportional to 1/sqrt(n). Dramatic improvements in reliability were seen using up to 25 minutes of imaging time, with smaller improvements for additional time. Functional connectivity “fingerprints” for the individual studied and the population began diverging at about 15 minutes imaging time with increasing reliability even at 4 hours of imaging time. A classifier discriminating scans during which our subject was resting or watching cartoons was 95% accurate at 10 minutes and 100% accurate at 15 minutes imaging time. An individual subject and control population converged to reliable, different functional connectivity profiles that were task-modulated and could be discriminated with sufficient imaging time. We suggest that quantitative single-subject diagnostic testing or classification use at least 25 minutes of BOLD imaging time if feasible.