Organ culture of craniofacial primordia
Organ culture of craniofacial primordia
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DOI：
10.1006/meth.2001.1156
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发表时间：
2001-05-01
期刊：
影响因子：
4.8
通讯作者：
Helms, J
中科院分区：
文献类型：
--
作者：
Hu, D;Helms, J
器官培养系统已被证明是研究正常和异常发育过程的非常有用的技术。将组织移植到器官培养系统中是少数几种在同时允许体外受控实验操作的条件下维持三维细胞相互作用的技术之一。在本文中，我们概述了在文化中培养“面孔”的程序。在该系统中，从胚胎小鼠或小鸡制备的面部原基可以在培养物中维持长达7-8天。在此期间，面部原基继续生长，分化，融合并发育成与原位观察到的结构非常相似的组织结构。该过程相对简单，只需要稳定的基质培养基，充分的氧合和在37 ℃下孵育器官系统。该程序的优点和缺点，沿着详细的方法，以帮助解决一些常见的陷阱器官培养系统。(C)北京：科学出版社.
Organ culture systems have proven extremely useful techniques in studies that investigate the process of normal and abnormal development. The explant of tissues into an organ culture system is one of the few techniques that maintains three-dimensional cellular interactions under conditions that simultaneously permit controlled experimental manipulation In vitro. In this article we outline a procedure for growing "faces" in culture. In this system, the facial primordia prepared from embryonic mice or chicks can be maintained in culture for up to 7-8 days. During this time, the facial primordia continue to grow, differentiate, fuse and develop into organized structures that closely resemble those observed in situ. The procedure is relatively simple, requiring only a stable substratum culture medium, sufficient oxygenation and incubation of the organ system at 37 degreesC. The advantages and disadvantages of the procedure are presented, along with detailed methods to help troubleshoot some of the common pitfalls of organ culture systems. (C) 2001 Academic Press.