T-CELL DEVELOPMENT DURING AGING IN CONGENITALLY ATHYMIC (NUDE) RATS
T-CELL DEVELOPMENT DURING AGING IN CONGENITALLY ATHYMIC (NUDE) RATS
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DOI：
10.1111/j.1365-3083.1986.tb02089.x
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发表时间：
1986-08-01
影响因子：
3.7
通讯作者：
SCHUURMAN, HJ
中科院分区：
文献类型：
--
作者：
VAESSEN, LMB;BROEKHUIZEN, R;SCHUURMAN, HJ
我们研究了WAG （RT-1u）系先天性胸腺缺陷（裸）大鼠在2、3、5、9和17月龄时的胸腺依赖性免疫系统。这包括脾脏、淋巴结和Peyer' s斑块的组织学，组织切片的免疫组织化学，细胞悬液的免疫细胞学，使用一组针对t淋巴细胞亚群的单克隆抗体，体外有丝分裂原反应性和体内对卵清蛋白免疫的反应性。结果与同年龄的免疫正常的窝友进行了比较。细胞标记分析，特别是在裸动物中，由于一些抗体对非t细胞的染色（对假定的巨噬细胞、自然杀伤细胞、b淋巴细胞系和髓系细胞的染色）而受到阻碍。尽管如此，随着年龄的增长，观察到这些标记细胞的增加；例如，在脾悬液中，经泛t试剂MRC OX-19标记的细胞从2个月时的5%增加到17个月时的19%（正常动物的值为34%）。在其他的实验中，也观察到t细胞的反应性随着年龄的增长而逐渐增加，从2个月和3个月大的裸鼠几乎没有t细胞反应，到17个月大的裸鼠的t细胞反应性大约是正常大鼠的一半。然而，没有一只裸动物对卵清蛋白免疫有反应。这些结果表明，在裸动物中发生的过程弥补了胸腺内t细胞的缺失。这尤其适用于t细胞表型和有丝分裂原反应性的变化，但不适用于抗原反应性。
We studied the thymus-dependent immune system in congenitally athymic (nude) rats of the WAG (RT-1u) strain, at the ages of 2, 3, 5, 9, and 17 months. This included the histology of spleen, lymph nodes, and Peyer''s patches, immunohistochemistry on tissue sections, and immunocytology on cell suspensions using a panel of monoclonal antibodies to T-lymphocyte subpopulations, in vitro mitogen responsiveness, and in vivo responsiveness to ovalbumin immunization. The results were compared with those in euthymic immunocompetent litter-mates of the same age. The cell marker analysis, especially in nude animals, was hampered by the staining of non-T cells by some of the antibodies (staining of putative macrophages, natural killer cells, cells of B-lymphocyte lineage, and of myeloid lineage). Despite this, with age an increase in cells with these markers was observed; for instance, in spleen suspensions, cells labelled by the pan-T reagent MRC OX-19 increased from 5% at 2 months to 19% at 17 months (value in euthymic animals 34%). In the other assays too a gradual increase of T-cell reactivity with age was observed, from almost absent in 2- and 3-month-old nude rats to values in 17-month-old animals of about half the level found in euthymic rats. However, none of the nude animals responded to ovalbumin immunization. These results indicate that in nude animals processes occur which compensate for the absent intrathymic T-cell generation. This applies in particular to changes in T-cell phenotype and mitogen responsiveness, but not to antigen responsiveness.