Expression of Neuropeptide Y, Omentin and Visfatin in Visceral and Subcutaneous Adipose Tissues in Humans: Relation to Endocrine and Clinical Parameters
Expression of Neuropeptide Y, Omentin and Visfatin in Visceral and Subcutaneous Adipose Tissues in Humans: Relation to Endocrine and Clinical Parameters
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DOI：
10.1159/000319508
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发表时间：
2010-01-01
期刊：
影响因子：
3.6
通讯作者：
Knerr, Ina
中科院分区：
文献类型：
--
作者：
Barth, Stephanie;Klein, Peter;Knerr, Ina
目的：探讨成人脂肪组织中神经肽Y （NPY）、网膜蛋白（omentin）和脂肪蛋白（visfatin）的表达与临床参数和激素的关系。方法：168例成人患者（手术肥胖患者31例，手术对照组31例，非手术肥胖患者76例，非手术对照组30例）。我们测量了血浆NPY（放射免疫法）、皮质醇（电化学发光免疫法）和尿液皮质醇代谢物（气相色谱/质谱法）。实时荧光定量PCR检测手术患者皮下和内脏脂肪组织标本中NPY、网膜蛋白和内脏脂肪蛋白的表达。结果：脂肪组织标本中检测到NPY，并且与血浆NPY浓度一样，组间具有可比性。Omentin基因在内脏组织中的表达高于皮下脂肪组织（p < 0.0001）。肥胖患者皮下组织Visfatin表达低于对照组（p < 0.05）。肥胖成人的皮质醇较对照组低（136.5 +/- 74.1 vs. 162.2 +/- 56.1 ng/ml; p < 0.05），各组间皮质醇代谢物具有可比性。结论：在我们的肥胖成人中，血浆NPY水平和糖皮质激素水平没有升高。尽管肥胖个体和对照组之间NPY、omentin和visfatin的表达相当，但我们必须考虑脂肪组织源性因子总生成速率的差异。
Objective: We aimed at exploring the expression of neuropeptide Y (NPY), omentin and visfatin in adipose tissues of adults along with clinical parameters and hormones. Methods: We included 168 adult patients (31 surgical obese patients and 31 surgical controls, 76 non-surgical obese patients, 30 non-surgical controls). We measured plasma NPY (by radioimmunoassay), cortisol (with an electrochemiluminescence immunoassay) and urinary cortisol metabolites (by gas chromatography/mass spectrometry). Expression of NPY, omentin and visfatin in subcutaneous and visceral adipose tissue specimens of the surgical patients was quantified using real-time PCR. Results: NPY was detectable in adipose tissue specimens and, like plasma NPY concentrations, comparable between groups. Omentin gene expression was higher in visceral than in subcutaneous adipose tissues (p < 0.0001). Visfatin expression was lower in the subcutaneous tissue of obese patients compared with controls (p < 0.05). Cortisol was lower in obese adults compared with controls (136.5 +/- 74.1 vs. 162.2 +/- 56.1 ng/ml; p < 0.05), cortisol metabolites were comparable between groups. Conclusion: In our obese adults, plasma NPY levels and the glucocorticoid measures were not elevated. Even though the expression of NPY, omentin and visfatin was comparable between obese individuals and controls, we have to consider differences in the total production rate of adipose tissue-derived factors.