Significant prognostic values of aquaporin mRNA expression in breast cancer
Significant prognostic values of aquaporin mRNA expression in breast cancer
复制标题
水通道蛋白 mRNA 表达在乳腺癌中的重要预后价值
DOI：
10.2147/cmar.s193396
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Ren, Yu
中科院分区：
文献类型：
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作者：
Zhu, Lizhe;Ma, Nan;Ren, Yu
水通道蛋白（Aquaporins，AQP），又称水通道，在乳腺癌细胞的迁移、侵袭和增殖中起重要作用。大多数AQP mRNA表达水平通过细胞系、小鼠模型甚至人类乳腺组织进行测试。然而，AQP在不同临床病理特征的乳腺癌患者中的表达及对不同类型乳腺癌患者的预后价值尚不清楚。材料与方法在本研究中，我们使用Oncomine数据库，乳腺癌基因表达Miner v4. 1，Kaplan-Meier Plotter和cBioPortal研究乳腺癌患者中AQP的表达分布和预后价值。结果乳腺癌组织中AQP 8、AQP 9和AQP 10的mRNA表达上调，AQP 3、AQP 4、AQP 5和AQP 7的mRNA表达下调。临床数据库显示，较低的AQP 1 mRNA水平与高Scarff-Bloom-Richardson分级相关，但AQP 9表现出相反的趋势。进一步的生存分析表明，AQP 0、AQP 1、AQP 2、AQP 4、AQP 6、AQP 8、AQP 10和AQP 11的高mRNA表达水平与较好的无复发生存率（RFS）显著相关。相反，AQP 3和AQP 9与乳腺癌患者更差的RFS相关，这表明这两个基因可能是未来化疗的潜在靶点。这些重要的AQP成员可能作为乳腺癌预后的新的生物标志物进一步探索，但这需要进一步研究。
Introduction Aquaporins (AQPs), also called water channels, have been shown to have functions in the migration, invasion, and proliferation of human breast tumor cells. Most AQP mRNA expression levels were tested by cell lines, mouse models, and even human breast tissues. However, the mRNA expression of individual AQPs in different clinicopathologic characteristics and prognostic values according to different kinds of classifications of breast cancer patients remains unclear. Materials and methods In the current study, we used the Oncomine database, Breast cancer Gene-Expression Miner v4.1, Kaplan–Meier Plotter, and cBioPortal to investigate the expression distribution and prognostic values of AQPs in breast cancer patients. Results Our study revealed that the mRNA expression levels of AQP8, AQP9, and AQP10 were upregulated, while those of AQP3, AQP4, AQP5, and AQP7 were downregulated in breast cancer. The clinical database showed that lower mRNA levels of AQP1 were associated with a high Scarff–Bloom–Richardson grade, but AQP9 showed the opposite trend. Further survival analyses indicated that high mRNA expression levels of AQP0, AQP1, AQP2, AQP4, AQP6, AQP8, AQP10, and AQP11 were significantly associated with better relapse-free survival (RFS). Conversely, AQP3 and AQP9 were associated with worse RFS in breast cancer patients, suggesting that these two genes might be potential targets in future chemotherapy. Discussion These significant AQP members might be further explored as new biomarkers for breast cancer prognosis, but this needs further study.